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PDGF-BB Homodimer (AA 74-182) (Active) Protéine

Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
N° du produit ABIN2018325
  • Antigène
    PDGF-BB Homodimer
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 74-182
    Origine
    • 10
    • 5
    • 2
    Rat
    Source
    • 12
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Attributs du produit
    ED50 < 2 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 5 x 10^5 units/mg.
    AA 74-182, expressed with an N-terminal Met.
    Pureté
    > 95 % by SDS-PAGE analysis.
    niveau d'endotoxine
    < 0.2 EU/μg, determined by LAL method.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstituted in 20 mM acetic acid at 100 μg/mL.
    Buffer
    Lyophilized after extensive dialysis against 20 mM acetic acid.
    Stock
    -80 °C
    Stockage commentaire
    Lyophilized recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrPDGF-BB remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
    Date de péremption
    6 months
  • Antigène
    PDGF-BB Homodimer
    Autre désignation
    Platelet-Derived Growth Factor-BB (PDGF-BB)
    Synonymes
    PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, Pdgfb Protein
    Sujet
    Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rrPDGF-BB has a molecular mass of 24.6 kDa analyzed by non-reducing SDS-PAGE.
    Synonyms: Platelet-Derived Growth Factor-BB, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
    Poids moléculaire
    24.6 kDa, observed by non-reducing SDS-PAGE.
    UniProt
    Q05028
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