CD19 Protein (AA 20-291) (Fc Tag)

N° du produit ABIN2180715

Détail du produit

Antigène: CD19 (CD19 Molecule (CD19))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 20-291

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD19 protéine est marqué à la Fc Tag.

Séquence: AA 20-291

Attributs du produit: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 56.3 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.1 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Référence

Hsu, Boudova, Mvula, Divala, Mungwira, Harman, Laufer, Pauza, Cairo: "Prolonged PD1 Expression on Neonatal Vδ2 Lymphocytes Dampens Proinflammatory Responses: Role of Epigenetic Regulation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 197, Issue 5, pp. 1884-92, (2017) (PubMed).

De Oliveira, Wang, Ryan, Morrison, Kohn, Hollis: "A CD19/Fc fusion protein for detection of anti-CD19 chimeric antigen receptors." dans: Journal of translational medicine, Vol. 11, pp. 23, (2013) (PubMed).

Détail du antigène

Antigène: CD19 (CD19 Molecule (CD19))

Autre désignation: CD19 (CD19 Produits)

Synonymes: B4 Protein, CVID3 Protein, AW495831 Protein, CD19 Protein, CD19 molecule Protein, CD19 antigen Protein, CD19 Protein, Cd19 Protein

Sujet: B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I  membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.

Poids moléculaire: 56.3 kDa

NCBI Accession: NP_001761

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway