CD4 Protein (CD4) (AA 26-396) (Fc Tag)

N° du produit ABIN2180789

Détail du produit

Antigène: CD4

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 26-396

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD4 protéine est marqué à la Fc Tag.

Séquence: AA 26-396

Attributs du produit: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 67.5 kDa. The protein migrates as 80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Tris, Glycine and NaCl,  pH 7.5

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: CD4

Autre désignation: CD4 (CD4 Produits)

Synonymes: cd4 Protein, L3T4 Protein, Ly-4 Protein, CD4mut Protein, W3/25 Protein, p55 Protein, CD4 molecule Protein, carcinoembryonic antigen-related cell adhesion molecule 5 Protein, T-cell surface glycoprotein CD4 Protein, CD4 antigen Protein, Cd4 molecule Protein, CD4 Protein, LOC100125525 Protein, cd4 Protein, Cd4 Protein

Sujet: Cluster of Differentiation 4 (CD4) is also known as T-cell surface antigen T4/Leu-3 (LEU-3) and CD4mut, is a single-pass type I  membrane glycoprotein,and is a member of the immunoglobulin superfamily. CD4 expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It has four immunoglobulin domains (D1 to D4) that are exposed on the extracellular surface of the cell: D1 and D3 resemble immunoglobulin variable (IgV) domains. D2 and D4 resemble immunoglobulin constant (IgC) domains. CD4 is a co-receptor that assists the T cell receptor (TCR) with an antigen-presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR by recruiting an enzyme, known as the tyrosine kinase lck, which is essential for activating many molecules involved in the signaling cascade of an activated T cell. CD4 also interacts directly with MHC class II molecules on the surface of the antigen-presenting cell using its extracellular domain. The extracellular domain adopts an immunoglobulin-like beta-sandwich with seven strands in 2 beta sheets, in a Greek key topology. CD4 has also been shown to interact with SPG21, Lck and Protein unc-119 homolog. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

Poids moléculaire: 67.5 kDa

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events