CD55 Protein (AA 35-353) (His tag)

N° du produit ABIN2180811

Détail du produit

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Type de proteíne: Recombinant

Attributs du protein: AA 35-353

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD55 protéine est marqué à la His tag.

Séquence: AA 35-353

Attributs du produit: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.8 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Référence

Kwon, Kim, Meyer, Di Bisceglie, Ray: "Distinct CD55 Isoform Synthesis and Inhibition of Complement-Dependent Cytolysis by Hepatitis C Virus." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 197, Issue 4, pp. 1127-36, (2017) (PubMed).

Détail du antigène

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Autre désignation: CD55 (CD55 Produits)

Synonymes: CR Protein, CROM Protein, DAF Protein, TC Protein, Daf Protein, Daf-GPI Protein, Daf1 Protein, GPI-DAF Protein, CD55 molecule (Cromer blood group) Protein, CD55 molecule, decay accelerating factor for complement Protein, CD55 Protein, Cd55 Protein

Sujet: CD55 is also known as DAF or decay-accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologically-generated C3 convertases with its second and third SCRs. Binding results in an accelerated ""decay"", or dissociation of active C3 convertases, thus blocking the development of C' attack complexes on nonforeign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.

Poids moléculaire: 35.8 kDa

NCBI Accession: NP_000565

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity