EGFR Protein (AA 25-645) (His tag)

N° du produit ABIN6253725

Détail du produit

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 25-645

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette EGFR protéine est marqué à la His tag.

Séquence: AA 25-645

Attributs du produit: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 69.5 kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

Référence

Haueis, Stech, Kubick: "A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies." dans: Frontiers in bioengineering and biotechnology, Vol. 10, pp. 896763, (2022) (PubMed).

Hu, Wang, Jin, Liu, Cui, Sun, Li, Li, Wang, Zhen, Miao, Li: "An EGFR-targeting antibody-drug conjugate LR004-VC-MMAE: potential in esophageal squamous cell carcinoma and other malignancies." dans: Molecular oncology, Vol. 13, Issue 2, pp. 246-263, (2019) (PubMed).

Kahl, Settele, Knick, Haupts, Bosse-Doenecke: "Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts." dans: Protein expression and purification, Vol. 149, pp. 51-65, (2018) (PubMed).

Fazekas-Singer, Singer, Ilieva, Matz, Herrmann, Spillner, Karagiannis, Jensen-Jarolim: "AllergoOncology: Generating a canine anticancer IgE against the epidermal growth factor receptor." dans: The Journal of allergy and clinical immunology, Vol. 142, Issue 3, pp. 973-976.e11, (2018) (PubMed).

Fazekas-Singer, Berroterán-Infante, Rami-Mark, Dumanic, Matz, Willmann, Andreae, Singer, Wadsak, Mitterhauser, Jensen-Jarolim: "Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials." dans: Oncotarget, Vol. 8, Issue 47, pp. 83128-83141, (2017) (PubMed).

Yu, Pegram, Bigner, Chandramohan: "Development and validation of a cell-based fluorescent method for measuring antibody affinity." dans: Journal of immunological methods, Vol. 442, pp. 49-53, (2017) (PubMed).

Nath, Flemming, Godat, Urh: "Development of NanoLuc bridging immunoassay for detection of anti-drug antibodies." dans: Journal of immunological methods, Vol. 450, pp. 17-26, (2017) (PubMed).

Kant, Agarwal, Pancholi, Pancholi: "The Streptococcus pyogenes orphan protein tyrosine phosphatase, SP-PTP, possesses dual specificity and essential virulence regulatory functions." dans: Molecular microbiology, Vol. 97, Issue 3, pp. 515-40, (2016) (PubMed).

Zinn, Korb, Samuel, Warram, Dion, Killingsworth, Fan, Schoeb, Strong, Rosenthal: "IND-directed safety and biodistribution study of intravenously injected cetuximab-IRDye800 in cynomolgus macaques." dans: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 17, Issue 1, pp. 49-57, (2015) (PubMed).

Olsen, Jakobsen, Brandslund: "Quantification of EGFR autoantibodies in the amplification phenomenon of HER2 in breast cancer." dans: Clinical chemistry and laboratory medicine, Vol. 51, Issue 12, pp. 2325-9, (2014) (PubMed).

Singer, Fazekas, Wang, Weichselbaumer, Matz, Mader, Steinfellner, Meitz, Mechtcheriakova, Sobanov, Willmann, Stockner, Spillner, Kunert, Jensen-Jarolim: "Generation of a canine anti-EGFR (ErbB-1) antibody for passive immunotherapy in dog cancer patients." dans: Molecular cancer therapeutics, Vol. 13, Issue 7, pp. 1777-90, (2014) (PubMed).

Détail du antigène

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGF R (EGFR Produits)

Synonymes: C-erb Protein, CG10079 Protein, D-EGFR Protein, D-Egf Protein, DEGFR Protein, DER Protein, DER flb Protein, DER/EGFR Protein, DER/faint little ball Protein, DER/top Protein, DER/torpedo Protein, DER1 Protein, DEgfr Protein, Degfr Protein, Der Protein, DmHD-33 Protein, Dmel\\CG10079 Protein, EFG-R Protein, EGF-R Protein, EGFR Protein, EGFr Protein, EGfr Protein, EK2-6 Protein, Egf Protein, Egf-r Protein, EgfR Protein, El Protein, Elp Protein, Elp-1 Protein, Elp-B1 Protein, Elp-B1RB1 Protein, HD-33 Protein, TOP Protein, Torpedo/DER Protein, Torpedo/Egfr Protein, c-erbB Protein, d-egf-r Protein, dEGFR Protein, dEGFR1 Protein, dEgfr Protein, der Protein, egfr Protein, flb Protein, l(2)05351 Protein, l(2)09261 Protein, l(2)57DEFa Protein, l(2)57EFa Protein, l(2)57Ea Protein, mor1 Protein, top Protein, top/DER Protein, top/flb Protein, torpedo/Egfr Protein, torpedo/egfr Protein, EGFR12 Protein, EGFR15 Protein, egfr1 Protein, Erbb2 Protein, ERBB Protein, ERBB1 Protein, HER1 Protein, PIG61 Protein, mENA Protein, ErbB-1 Protein, Errp Protein, 9030024J15Rik Protein, AI552599 Protein, Erbb Protein, Errb1 Protein, Wa5 Protein, wa-2 Protein, wa2 Protein, epidermal growth factor receptor Protein, Epidermal growth factor receptor Protein, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Protein, EGFR Protein, Egfr Protein, egfra Protein, egfr1 Protein, LOC5564544 Protein

Sujet: The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Poids moléculaire: 70.5kDa

NCBI Accession: NP_005219

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins