FcRn Protein (AA 23-298) (His tag,Strep Tag)

N° du produit ABIN2181083

Détail du produit

Antigène: FcRn (IgG receptor FcRn (FcRn))

Type de proteíne: Recombinant

Attributs du protein: AA 23-298

Origine: Rat

Source: HEK-293 Cells

Purification/Conjugué: Cette FcRn protéine est marqué à la His tag,Strep Tag.

Marque: MABSol®

Séquence: AA 23-298

Attributs du produit: Rat FcRn / FCGRT & B2M Heterodimer Protein, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.8 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The predicted N-terminus is Ala 23 (FCGRT) & Ile 21 (B2M). The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14.5 kDa (B2M) respectively due to different glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Référence

Kenniston, Taylor, Conley, Cosic, Kopacz, Lindberg, Comeau, Atkins, Bullen, TenHoor, Adelman, Sexton, Edwards, Nixon: "Structural basis for pH-insensitive inhibition of immunoglobulin G recycling by an anti-neonatal Fc receptor antibody." dans: The Journal of biological chemistry, Vol. 292, Issue 42, pp. 17449-17460, (2017) (PubMed).

Détail du antigène

Antigène: FcRn (IgG receptor FcRn (FcRn))

Autre désignation: FcRn (FcRn Produits)

Sub Type: Fusionprotein

Sujet: FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Poids moléculaire: 31.8 kDa (FCGRT) & 13.1 kDa (B2M)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process