PCSK9 Protein (AA 31-692) (His tag)

N° du produit ABIN2181580

Détail du produit

Antigène: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 31-692

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette PCSK9 protéine est marqué à la His tag.

Séquence: AA 31-692

Attributs du produit: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 75.1 kDa. The protein migrates as 20 kDa and 62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.

Pureté: >97 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Référence

Burnap, Sattler, Pechlaner, Duregotti, Lu, Theofilatos, Takov, Heusch, Tsimikas, Fernandez-Hernando, Berry, Hall, Notdurfter, Rungger, Paulweber, Willeit, Kiechl, Levkau, Mayr: "PCSK9 Activity Is Potentiated Through HDL Binding." dans: Circulation research, (2021) (PubMed).

Chen, Lichtor, Berliner, Chen, Liu: "Evolution of sequence-defined highly functionalized nucleic acid polymers." dans: Nature chemistry, Vol. 10, Issue 4, pp. 420-427, (2019) (PubMed).

Lichtor, Chen, Elowe, Chen, Liu: "Side chain determinants of biopolymer function during selection and replication." dans: Nature chemical biology, Vol. 15, Issue 4, pp. 419-426, (2019) (PubMed).

Wijers, Zanoni, Liv, Vos, Jäckstein, Smit, Wilbrink, Wolters, van der Veen, Huijkman, Dekker, Kloosterhuis, van Dijk, Billadeau, Kuipers, Klumperman, von Eckardstein, Kuivenhoven, van de Sluis: "The hepatic WASH complex is required for efficient plasma LDL and HDL cholesterol clearance." dans: JCI insight, Vol. 4, Issue 11, (2019) (PubMed).

Grin, Dwivedi, Chathely, Trigatti, Prat, Seidah, Liaw, Fox-Robichaud: "Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor." dans: Scientific reports, Vol. 8, Issue 1, pp. 10496, (2018) (PubMed).

Dugardin, Briand, Touche, Schonewille, Moreau, Le May, Groen, Staels, Lestavel: "Retrograde cholesterol transport in the human Caco-2/TC7 cell line: a model to study trans-intestinal cholesterol excretion in atherogenic and diabetic dyslipidemia." dans: Acta diabetologica, Vol. 54, Issue 2, pp. 191-199, (2017) (PubMed).

Susan-Resiga, Girard, Kiss, Essalmani, Hamelin, Asselin, Awan, Butkinaree, Fleury, Soldera, Dory, Baass, Seidah: "The Proprotein Convertase Subtilisin/Kexin Type 9-resistant R410S Low Density Lipoprotein Receptor Mutation: A NOVEL MECHANISM CAUSING FAMILIAL HYPERCHOLESTEROLEMIA." dans: The Journal of biological chemistry, Vol. 292, Issue 5, pp. 1573-1590, (2017) (PubMed).

Topchiy, Cirstea, Kong, Boyd, Wang, Russell, Walley: "Lipopolysaccharide Is Cleared from the Circulation by Hepatocytes via the Low Density Lipoprotein Receptor." dans: PLoS ONE, Vol. 11, Issue 5, pp. e0155030, (2017) (PubMed).

Boyd, Fjell, Russell, Sirounis, Cirstea, Walley: "Increased Plasma PCSK9 Levels Are Associated with Reduced Endotoxin Clearance and the Development of Acute Organ Failures during Sepsis." dans: Journal of innate immunity, Vol. 8, Issue 2, pp. 211-20, (2016) (PubMed).

Walley, Thain, Russell, Reilly, Meyer, Ferguson, Christie, Nakada, Fjell, Thair, Cirstea, Boyd: "PCSK9 is a critical regulator of the innate immune response and septic shock outcome." dans: Science translational medicine, Vol. 6, Issue 258, pp. 258ra143, (2015) (PubMed).

Détail du antigène

Antigène: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Autre désignation: PCSK9 (PCSK9 Produits)

Synonymes: FH3 Protein, HCHOLA3 Protein, LDLCQ1 Protein, NARC-1 Protein, NARC1 Protein, PC9 Protein, AI415265 Protein, AI747682 Protein, Narc1 Protein, proprotein convertase subtilisin/kexin type 9 Protein, proprotein convertase subtilisin/kexin type 9 L homeolog Protein, PCSK9 Protein, pcsk9.L Protein, pcsk9 Protein, Pcsk9 Protein

Sujet: Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Poids moléculaire: 13.8 kDa and 58.1 kDa