SPAM1 Protein (AA 36-482) (His tag)

N° du produit ABIN2181785

Détail du produit

Antigène: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Type de proteíne: Recombinant

Attributs du protein: AA 36-482

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette SPAM1 protéine est marqué à la His tag.

Séquence: AA 36-482

Attributs du produit: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.1 kDa. The protein migrates as 64-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM NaCl, pH 7.5

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Détail du antigène

Antigène: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Autre désignation: PH20 (SPAM1 Produits)

Synonymes: AV039194 Protein, Ph-20 Protein, PH-20 Protein, PH20 Protein, HYA1 Protein, HYAL1 Protein, HYAL3 Protein, HYAL5 Protein, SPAG15 Protein, Spam Protein, XEH1 Protein, spam1 Protein, im:6902904 Protein, zgc:158668 Protein, sperm adhesion molecule 1 Protein, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) L homeolog Protein, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) Protein, Spam1 Protein, SPAM1 Protein, spam1.L Protein, spam1 Protein

Sujet: Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N - acetyl - beta - D - glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Poids moléculaire: 53.0 kDa

NCBI Accession: NP_694859