Sirtuin 6 (SIRT6) (Active) protein (GST tag)

Détails pour le produit réf. ABIN2506866, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • 2810449N18Rik
  • AI043036
  • Sir2l6
  • SIR2L6
  • zgc:86729
  • sirtuin 6
  • sirtuin 6 S homeolog
  • Sirt6
  • SIRT6
  • sirt6.S
  • sirt6
Origine
Humain
14
4
2
Source
Escherichia coli (E. coli)
13
2
2
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
GST tag
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Options
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N° du produit (Fournisseur)
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Attributs du produit Molecule Information: Human SIRT6 is a 65.3 kDa (1-355 aa + N-terminal GST tag) protein. It runs as ~62 kDa on a SDS-PAGE.
Pureté ≥85 % by SDS-PAGE
Biological Activity Comment >3 mU/mg

Specification / Test Method: Active GST-tagged SIRT6 has been tested for its activity using Sirtuin Activity Assay Kit. It has a specific activity of >3 mU/mg (One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 μmol of AFC/min at 37°C).
Unit Definition One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 μmol of AFC/min at 37°C
Nom de la protéine
Sujet Human Sirtuins function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Sirtuin 6 (SIRT6) is a NAD-dependent protein deacetylase with activity towards Lys-9 and Lys-56 of histone H3. It modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. It is required for genomic stability and for normal IGF1 serum levels and normal glucose homeostasis. It also regulates cellular senescence and apoptosis. Unlike most sirtuins, SIRT6 preferentially removes long-chain fatty-acyl, rather than acetyl, protein modifications. This activity regulates secretion of the inflammation-associated protein TNF-α.

Alternative Names: SIRT6, NAD-dependent Deacetylase Sirtuin-6, Mono-ADP-ribosyltransferase Sirtuin 6, SIR2-like Protein 6, SIR2L6
ID gène 51548
UniProt Q8N6T7
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Human SIRT6 protein is a solution of 1 mg/mL in 50 mM Tris, 100 mM NaCl, pH 8.0 containing 20 % glycerol.
Conseil sur la manipulation Centrifuge the vial prior to opening.
Stock -80 °C
Date de péremption 12 months
Images (Fournisseur)
Activity Assay (AcA) image for Sirtuin 6 (SIRT6) (Active) protein (GST tag) (ABIN2506866) Sirtuin 6 (SIRT6) (Active) protein (GST tag)
Western Blotting (WB) image for Sirtuin 6 (SIRT6) (Active) protein (GST tag) (ABIN2506866) Sirtuin 6 (SIRT6) (Active) protein (GST tag) (Image 2)
Activity Assay (AcA) image for Sirtuin 6 (SIRT6) (Active) protein (GST tag) (ABIN2506866) Sirtuin 6 (SIRT6) (Active) protein (GST tag) (Image 3)
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