C-Type Lectin Domain Family 1, Member B (CLEC1B) (AA 157-229), (C-Term) (Active) protein (Fc Tag)

N° du produit ABIN2666791

Détail du produit

Antigène: C-Type Lectin Domain Family 1, Member B (CLEC1B)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 157-229, C-Term

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Fc Tag

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Pureté: > 95 % , as determined by Coomassie stained SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 1.0 EU per μg of protein as determine by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: Recombinant mouse CLEC2, when coated on a plate (2 μg/ml, 100 μl/well) is able to bind biotinylated mouse GP38 in a dose dependent manner with an apparent kD <5.0 nM.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Concentration: 200 μg/mL

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: C-Type Lectin Domain Family 1, Member B (CLEC1B)

Autre désignation: CLEC2 (CLEC1B Produits)

Synonymes: 1810061I13Rik Protein, CLEC2 Protein, CLEC2B Protein, PRO1384 Protein, QDED721 Protein, Clec-2 Protein, Clec2 Protein, CLEC1B Protein, RGD1563517 Protein, CLEC9A Protein, C-type lectin domain family 1 member B Protein, C-type lectin domain family 1, member b Protein, C-type lectin domain family 1, member B Protein, CLEC1B Protein, Clec1b Protein

Classe de substances: Viral Protein

Sujet: CLEC2, also termed CLEC-1B, is a C-type lectin-like receptor. CLEC2 is highly expressed on resting platelets and megakaryocytes, and expressed at lower levels on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. The first ligand identified for CLEC2 was rhodocytin, snake venom toxin. Rhodocytin is a strong platelet activator, and this effect is mediated by CLEC2, which has a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (ITAM). Exposure of platelets to rhodocytin leads to tyrosine phosphorylation of the ITAM-like motif and Syk-dependent platelet activation. Podoplanin, also termed GP38 is an endogenous ligand of CLEC2. This molecule was found to be expressed on the surface of tumor cells and facilitates tumor metastasis by inducing platelet aggregation. Podoplanin is also expressed on endothelial cells, and mice deficient in this protein show abnormal patterns of lymphatic vessel formation. In addition, CLEC2 functions as an activating receptor on monoyctes and neutrophils to induce phagocytosis and proinflammatory cytokine production. CLEC2 enhances the expansion of TCR-stimulated T cells by increasing their survival through enhanced expression of anti-apoptotic proteins. Also, CLEC2 modulates the capacity of T cells to home to lymph nodes and stimulates natural killer cell mediated cytotoxicity.

Poids moléculaire: The 409 amino acid recombinant protein has a predicted molecular mass of approximately 46.7 kDa. The protein migrates at about 60 kDa by SDS-PAGE in DTT-reducing conditions and about 120 kDa in non-reducing conditions. The predicted N-terminal amino acid