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Podoplanin Protein (PDPN) (AA 12-137, C-Term) (Fc Tag)

PDPN Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % , as determined by Coomassie stained SDS-PAGE. FACS Active
N° du produit ABIN2666858
  • Antigène Voir toutes Podoplanin (PDPN) Protéines
    Podoplanin (PDPN)
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 12-137, C-Term
    Origine
    • 13
    • 11
    • 4
    • 2
    • 1
    • 1
    Souris
    Source
    • 13
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification/Conjugué
    Cette Podoplanin protéine est marqué à la Fc Tag.
    Application
    Flow Cytometry (FACS)
    Pureté
    > 95 % , as determined by Coomassie stained SDS-PAGE.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine

    Less than 0.1 EU per μg of protein as determine by the LAL method.

    Top Product
    Discover our top product PDPN Protéine
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Commentaires

    Biological activity: Recombinant mouse podoplanin, when coated on a plate (2 μg/ml, 100 μl/well), is able to bind mouse CLEC-2 in a dose dependent manner with an apparent kD <1.0 nM.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
    Concentration
    200 μg/mL
    Buffer
    0.22 μm filtered protein solution is in PBS.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
  • Antigène
    Podoplanin (PDPN)
    Autre désignation
    Podoplanin (PDPN Produits)
    Synonymes
    PDPN Protein, AGGRUS Protein, GP36 Protein, GP40 Protein, Gp38 Protein, HT1A-1 Protein, OTS8 Protein, PA2.26 Protein, T1A Protein, T1A-2 Protein, OTS-8 Protein, RANDAM-2 Protein, T1-alpha Protein, T1a Protein, T1alpha Protein, E11 Protein, RTI40 Protein, podoplanin Protein, PDPN Protein, Pdpn Protein
    Sujet
    Podoplanin, also termed GP38, is a 38 kD type I transmembrane sialoglycoprotein and a member of the podoplanin family. Mouse and human GP38 share 46 % amino acid sequence homology. Alternative splicing transcripts, encoding different isoforms of GP38, have been identified. Structurally, GP38 has a 119 amino acid extracellular domain (ECD), a 21 amino acid transmembrane region, and a 10 amino acid cytoplasmic tail. This type I protein is heavily O-glycosylated and has a diverse distribution in human tissues, including glomerular epithelial cells, lung alveolar cells, lymphatic endothelial cells, and neural tissues. GP38 is also expressed by tumor cells such as testicular seminomas, squamous cell carcinomas, colorectal tumors, and brain tumors. Therefore, this protein is a target of antibody therapy in cancer treatment. GP38 induces platelet aggregation and tumor metastasis through its association with CLEC2. The binding of GP38 and CLEC2 is important for maintaining high endothelial cell venule integrity. Also, GP38 is necessary for lymphatic vessel formation, normal lung cell proliferation, and alveolus formation at birth. In addition, GP38 was shown to be a novel marker for immature hepatic progenitor cells in fetal livers. GP38 is upregulated in Th17 cells in the arthritic joints in SKG mice, suggesting that this protein could be a useful Th17 cell marker for diagnosing pathological conditions of autoimmune diseases, including rheumatoid arthritis.
    Poids moléculaire
    This 362 amino acid recombinant protein predicted has a molecular mass of approximately 39.8 kDa. The protein migrates at about 60 kDa by SDS-PAGE in DTT-reducing conditions and about 130 kDa in non-reducing conditions. The predicted N-terminal amino acid
    Pathways
    Dicarboxylic Acid Transport
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