PRC2 EZH2 (full length), (Tyr641Asn-Mutant) protein (DYKDDDDK Tag)

N° du produit ABIN2669655

Détail du produit

Antigène: PRC2 EZH2

Type de proteíne: Recombinant

Attributs du protein: Tyr641Asn-Mutant, full length

Origine: Humain

Source: Baculovirus

Purification/Conjugué: DYKDDDDK Tag

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Attributs du produit: Recombinant PRC2 EZH2(Y641N) Complex that includes full length EZH2 with a Y641N mutation complexed with full length SUZ12, EED and RbAp46/48 (accession numbers NP_001190176.1, NP_056170, NP_003788.2, NP_002884.1, and NP_005601.1, respectively) was expressed in Sf9 and contains an N-terminal FLAG tag at the N-terminus of EZH2. The molecular weights of expressed EZH2(Y641N), SUZ12, EED and RbAp46/48 are 87 kDa, 83 kDa, 50.2 kDa, 47.8 kDa and 47.7 kDa, respectively.

Information d'application

Indications d'application: PRC2 EZH2(Y641N) Complex is suitable for use in the study of enzyme kinetics, inhibitor screening and selectivity profiling. Specific Activity: H3K27me3 and H3K27me2 methyltransferase.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Détail du antigène

Antigène: PRC2 EZH2

Sujet: PRC2 (Polycomb Repressive Complex 2) is one of the two classes Polycomb-group, or PcG proteins (the other being PRC1) that are important epigenetic determinants of stem cell identity. They play an important role in long-term epigenetic silencing of genes during cell fate determination and differentiation. PRC2 functions as a repressor of chromatin. PRC2 is required to target recruitment to specific DNA sequences (termed Polycomb Response Elements or PREs) of genomic regions to be silenced. Once associated with chromatin, the PRC2 subunit EZH2 has histone methyltransferase activity that catalyzes the trimethylation of histone H3 at Lys27. H3K27me3 is well established as a hallmark of regions of repressed chromatin. Trimethylation of Lys27 leads to the recruitment of PRC1 through the binding of H3K27me3 by chromodomain-containing proteins in PRC1. PRC1 is responsible for long-term gene silencing after cellular differentiation. Recombinant PRC2 EZH2(Y641N) Complex is a mutant version of our wild-type PRC2 Complex that contains a Tyr-to-Asn mutation at tyrosine 641 of the SET domain of EZH2. Somatic mutations of tyrosine 641, including Y641F, Y641N, Y641C, Y641S and Y641H, have been identified in patients with non-Hodgkin lymphoma and have been shown to alter substrate specificity and catalytic activity of EZH2 for histone H3 lysine 27 (H3K27) methylation states resulting in increased H3K27 trimethylation.

Poids moléculaire: The molecular weights of expressed EZH2(Y641N), SUZ12, EED and RbAp46/48 are 87 kDa, 83 kDa, 50.2 kDa, 47.8 kDa and 47.7 kDa, respectively.