Lysine (K)-Specific Demethylase 5A (KDM5A) protein (DYKDDDDK Tag)

Détails pour le produit réf. ABIN2669680, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • Jarid1a
  • JARID1A
  • RBBP-2
  • RBBP2
  • RBP2
  • AA409370
  • C76986
  • Rbbp2
  • lysine demethylase 5A
  • lysine (K)-specific demethylase 5A
  • Kdm5a
  • KDM5A
Origine
Humain
4
Source
Baculovirus
1
1
1
1
Type de proteíne
Recombinant
Purification/Conjugué
Cette KDM5A protéine est marqué à la DYKDDDDK Tag.
Application
Enzyme Activity Assay (EAA), Screening Assay (ScA)
Options
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N° du produit (Fournisseur)
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Attributs du produit Recombinant JARID1A / KDM5A (accession number NP_001036068.1) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 196.3 kDa.
Nom de la protéine
Sujet Lysine (K)-specific demethylase 5A (KDM5A), also known as Jumonji, AT rich interactive domain 1A (JARID1A), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5A demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5A may function to stimulate transcription mediated by nuclear receptors and may be involved in transcriptional regulation of HOX proteins during cell differentiation. Also, KDM5A may be involved in the transcriptional repression of cytokines such as CXCL12. It has been shown to bind directly with the Retinoblastoma (pRb) protein which regulates cell proliferation. KDM5A also interacts with Rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis.
Poids moléculaire 196.3 kDa
Pathways Chromatin Binding
Indications d'application Recombinant JARID1A / KDM5A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Catalytic rate: >60 turnovers/ enzyme molecule/ hour. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JARID1A / KDM5A protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
Restrictions For Research Use only
Conseil sur la manipulation Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Stock -80 °C
Stockage commentaire Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
Images (Fournisseur)
Activity Assay (AcA) image for Lysine (K)-Specific Demethylase 5A (KDM5A) protein (DYKDDDDK Tag) (ABIN2669680) JARID1A / KDM5A activity assay. Recombinant JARID1A / KDM5A activity measured using a...
Screening Assay (ScA) image for Lysine (K)-Specific Demethylase 5A (KDM5A) protein (DYKDDDDK Tag) (ABIN2669680) Recombinant JARID1A / KDM5A activity using AlphaLISA. JARID1A / KDM5A was used in an ...
Western Blotting (WB) image for Lysine (K)-Specific Demethylase 5A (KDM5A) protein (DYKDDDDK Tag) (ABIN2669680) Recombinant JARID1A / KDM5A protein gel. JARID1A / KDM5A protein was run on an SDS-PA...
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