Tumor Protein P53 (TP53) (Active) protein (His tag)

Détails pour le produit réf. ABIN2669713, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Origine
Humain
32
5
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
25
24
3
3
2
1
1
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
Cette p53 protéine est marqué à la His tag.
Application
In vitro Assay (in vitro), Protein Interaction (PI), Standard (STD)
Options
Fournisseur
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N° du produit (Fournisseur)
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Attributs du produit p53 is a tumor suppressor protein functioning as a sequence-specific transcription factor important for cell cycle arrest, apoptosis, and DNA-damage response. Recombinant p53, transcript variant 1, was expressed in E. coli from human cDNA (accession number NM_000546.5) with an N-terminal polyhistidine tag. Active Motif also offers Recombinant p53 protein (Catalog No. 31103) that was expressed in Baculovirus.
Nom de la protéine
Sujet P53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.
Pathways Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
Indications d'application Recombinant p53 is suitable for use in Active Motif's TransAM® assay as a protein standard, in vitro transcription assays, protein-protein interactions and protein-DNA interaction studies. A recommended starting point for in vitro transcription assays is 20-100 ng per reaction. The molecular weight of the protein is 53 kDa.
Restrictions For Research Use only
Conseil sur la manipulation Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Stock -80 °C
Stockage commentaire Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
Images (Fournisseur)
Western Blotting (WB) image for Tumor Protein P53 (TP53) (Active) protein (His tag) (ABIN2669713) Recombinant p53 protein analyzed by SDS-PAGE gel. SDS-PAGE analysis of 500 ng Recombi...
Western Blotting (WB) image for Tumor Protein P53 (TP53) (Active) protein (His tag) (ABIN2669713) Recombinant p53 protein analyzed by Western blot. Western blot image of 500 ng Recomb...
Activity Assay (AcA) image for Tumor Protein P53 (TP53) (Active) protein (His tag) (ABIN2669713) TransAM® standard curve generated using Recombinant p53 protein, active. The standard...
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