LIG1 Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2719545

Détail du produit

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette LIG1 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human DNA ligase 1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Liu, Lu, Ali, Liu, Zheng, Dai, Li, Xu, Hua, Zhou, Ortega, Li, Kunkel, Shen: "Okazaki fragment maturation involves α-segment error editing by the mammalian FEN1/MutSα functional complex." dans: The EMBO journal, Vol. 34, Issue 13, pp. 1829-43, (2015) (PubMed).

Détail du antigène

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Autre désignation: Dna Ligase 1 (LIG1 Produits)

Synonymes: ATLIG1 Protein, DNA ligase 1 Protein, T6D22.23 Protein, ligI Protein, AL033288 Protein, LigI Protein, lig1 Protein, wu:fc54a11 Protein, DNA ligase 1 Protein, DNA ligase 1, putative Protein, ligase I, DNA, ATP-dependent L homeolog Protein, ligase I, DNA, ATP-dependent Protein, LIG1 Protein, PB000674.02.0 Protein, PTRG_06243 Protein, PKH_140260 Protein, lig1.L Protein, Lig1 Protein, lig-1 Protein, lig1 Protein

Sujet: This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Poids moléculaire: 101.6 kDa

NCBI Accession: NP_000225

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA