IDO1 Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2723258

Détail du produit

Antigène: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette IDO1 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human IDO1 / INDO protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: The specific activity of IDOI was determined by monitoring kynurenine formation from N-formylkynurenine based on the absorbance at 492nm. The N-formylkynurenine was produced from a conversion of tryptophan with IDO1. The reaction was carried out at 25°C for 15min in the buffer containing 100mM PBS, pH6.5, 40mM ascorbic acid, 450 units catalase, 20μM methylene blue, and 800μM L-tryptophan as the substrate. The reaction was terminated by adding 50ul of 30% (w/v) trichloroacetic acid. The sample was further incubated for 30min at 60°C and centrifuged at 12000 rpm for 15 min. The supernatant was used to mix with an equal volume of Ehrlich's reagent (2% p-dimethylaminobenzaldehyde in glacial acetic acid) to measure the absorbance at 492 nm after 10min incubation.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Autre désignation: Ido1,indo (IDO1 Produits)

Synonymes: INDO Protein, ido Protein, indo Protein, IDO Protein, cd107b Protein, IDO1 Protein, IDO-1 Protein, Ido Protein, Indo Protein, indoleamine 2,3-dioxygenase 1 Protein, indoleamine 2,3-dioxygenase 1 L homeolog Protein, indoleamine 2,3-dioxygenase 1-like Protein, IDO1 Protein, ido1.L Protein, ido1 Protein, Ido1 Protein, LOC100050681 Protein

Sujet: This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011].

Poids moléculaire: 45.1 kDa

NCBI Accession: NP_002155

Pathways: Activated T Cell Proliferation