IFNB1 Protéine
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- Antigène Voir toutes IFNB1 Protéines
- IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Humain
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Source
- CHO Cells
- Application
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- Specificité
- Optimal preservation of protein structure, post-translational modifications and functions.
- Attributs du produit
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- Recombinant human IFNB / Interferon beta protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Pureté
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- niveau d'endotoxine
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Assay #1:Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/ml. Assay #2:Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is less than or equal to 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7 units/mg
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- Discover our top product IFNB1 Protéine
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- Indications d'application
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Conseil sur la manipulation
- Centrifuge the vial prior to opening. Reconstitute the protein in water to a concnetration of 0.1 ~ 1.0 mg/mL. Do not vertex. For extended storage, it is recommended to further dilute in a proper buffer (i.e. water or PBS) containing a carrier protein such as 0.1 % BSA, and store in working aliquots at -20 °C or -80 °C
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Antigène
- IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))
- Autre désignation
- Ifnb,interferon beta (IFNB1 Produits)
- Synonymes
- IFB Protein, IFF Protein, IFNB Protein, IFN-beta Protein, Ifb Protein, Ifnb Protein, IFNb Protein, interferon beta 1 Protein, interferon beta 1, fibroblast Protein, interferon, beta 1, fibroblast Protein, IFNB1 Protein, Ifnb1 Protein
- Sujet
- Has antiviral, antibacterial and anticancer activities. [UniProtKB/Swiss-Prot Function]
- Poids moléculaire
- 20 kDa
- NCBI Accession
- NP_002167
- Pathways
- Signalistation JAK/STAT, TCR Signaling, Signalisation TLR, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome
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