RUNX1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

N° du produit ABIN2731253

Détail du produit

Antigène: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Transcript Variant 2

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette RUNX1 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human RUNX1 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: RUNX1 Activity verified in a DNA-binding assay:

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Mochin, Underwood, Cooper, McLenithan, Pierce, Nalvarte, Arbiser, Karlsson, Moise, Moskovitz, Passaniti: "Hyperglycemia and redox status regulate RUNX2 DNA-binding and an angiogenic phenotype in endothelial cells." dans: Microvascular research, Vol. 97, pp. 55-64, (2014) (PubMed).

Détail du antigène

Antigène: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Autre désignation: Runx1 (RUNX1 Produits)

Synonymes: RUNX1 Protein, runx1 Protein, AI462102 Protein, AML1 Protein, Cbfa2 Protein, Pebp2a2 Protein, Pebpa2b Protein, AML1-EVI-1 Protein, AMLCR1 Protein, CBFA2 Protein, EVI-1 Protein, PEBP2aB Protein, runxa Protein, Runx-1 Protein, XAML Protein, Xaml1 Protein, aml Protein, aml-1 Protein, aml1 Protein, aml1-evi-1 Protein, amlcr1 Protein, cbfa2 Protein, evi-1 Protein, pebp2ab Protein, Aml1 Protein, uncharacterized LOC473981 Protein, runt-related transcription factor Protein, runt related transcription factor 1 Protein, runt-related transcription factor 1 Protein, runt related transcription factor 1 L homeolog Protein, LOC473981 Protein, runt Protein, Runx1 Protein, RUNX1 Protein, runx1 Protein, runx1.L Protein

Sujet: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 48.6 kDa

NCBI Accession: NP_001001890