SMAD4 Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2732222

Détail du produit

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette SMAD4 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human SMAD4 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Atanelishvili, Shirai, Akter, Buckner, Noguchi, Silver, Bogatkevich: "M10, a caspase cleavage product of the hepatocyte growth factor receptor, interacts with Smad2 and demonstrates antifibrotic properties in vitro and in vivo." dans: Translational research : the journal of laboratory and clinical medicine, Vol. 170, pp. 99-111, (2016) (PubMed).

Détail du antigène

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Autre désignation: Smad4 (SMAD4 Produits)

Synonymes: smad4 Protein, DPC4 Protein, JIP Protein, MADH4 Protein, MYHRS Protein, AW743858 Protein, D18Wsu70e Protein, Madh4 Protein, XSmad4alpha Protein, Xsmad4 Protein, dpc4 Protein, jip Protein, madh4 Protein, xsmad4a Protein, SMAD family member 4 Protein, Smad4 protein Protein, SMAD family member 4, gene 1 L homeolog Protein, SMAD4 Protein, smad4 Protein, Smad4 Protein, smad4.1.L Protein

Sujet: This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

Poids moléculaire: 60.3 kDa

NCBI Accession: NP_005350

Pathways: Cycle Cellulaire, Chromatin Binding, Autophagy