TNFSF15
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, PI, AbP, STD
Active
TNFSF15
(Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))
Type de proteíne
Recombinant
Activité biologique
Active
Attributs du protein
Transcript Variant 1
Origine
Humain
Source
Escherichia coli (E. coli)
Application
Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human TNFSF15 (transcript variant 1) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
> 95 % as determined by SDS-PAGE and Coomassie blue staining
niveau d'endotoxine
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Determined by its ability to stimulate IFN-gamma production by human PBMC using a concentration range of 10.0-100.0 ng/ml. Note: Results may vary with PBMC donors.
Crystallography grade
TNFSF15
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS, Crys
Indications d'application
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Conseil sur la manipulation
Resuspend the protein in the desired concentration in proper buffer
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Antigène
TNFSF15
(Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))
TTPAL Protein, TL1 Protein, TL1A Protein, VEGI Protein, VEGI192A Protein, Tl1 Protein, Tl1a Protein, Vegi Protein, tumor necrosis factor superfamily member 15 Protein, TNF superfamily member 15 Protein, tumor necrosis factor (ligand) superfamily, member 15 Protein, TNFSF15 Protein, Tnfsf15 Protein
Sujet
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Two transcript variants encoding different isoforms have been found for this gene.