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TNF alpha Protein (AA 77-233) (His tag,AVI tag,Biotin)

TNF alpha Origine: Humain Hôte: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
N° du produit ABIN2870564
  • Antigène Voir toutes TNF alpha Protéines
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 77-233
    Origine
    • 42
    • 24
    • 12
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 61
    • 28
    • 22
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification/Conjugué
    Cette TNF alpha protéine est marqué à la His tag,AVI tag,Biotin.
    Marque
    MABSol®,PrecisionAvi
    Séquence
    AA 77-233
    Specificité
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Attributs du produit
    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) The protein has a calculated MW of 19.2 kDa. The protein migrates as 21 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. More than 95 % of Biotinylated Human TNF-alpha (TNA-H82E3) presents as active trimers with a molecular mass of 57 kDa, as determined by SEC-HPLC analysis.
    Pureté
    >95 % as determined by SDS-PAGE.
    niveau d'endotoxine
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product TNF alpha Protéine
  • Commentaires

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Conseil sur la manipulation
    Please avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
  • Bourquard, Musnier, Puard, Tahir, Ayoub, Jullian, Boulo, Gallay, Watier, Bruneau, Reiter, Crépieux, Poupon: "MAbTope: A Method for Improved Epitope Mapping." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 201, Issue 10, pp. 3096-3105, (2019) (PubMed).

    Ji, Cao, Zeng, Zhang, Xiao, Guan, Chen, Chen, Wang, Guo: "The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway." dans: Journal of cellular biochemistry, Vol. 119, Issue 2, pp. 1979-1991, (2018) (PubMed).

    Karlsson, Fridh, Frostell: "Surrogate potency assays: Comparison of binding profiles complements dose response curves for unambiguous assessment of relative potencies." dans: Journal of pharmaceutical analysis, Vol. 8, Issue 2, pp. 138-146, (2018) (PubMed).

  • Antigène
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Autre désignation
    TNF-alpha (TNF alpha Produits)
    Synonymes
    DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
    Sujet
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
    Poids moléculaire
    20.0 kDa
    NCBI Accession
    NP_000585
    Pathways
    Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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