Streptavidin Protein (HRP)

N° du produit ABIN3041800

Détail du produit

Antigène: Streptavidin

Type de proteíne: Native

Origine: Streptomyces avidinii

Source: Streptomyces avidinii

Purification/Conjugué: Cette Streptavidin protéine est marqué à la HRP.

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Attributs du produit: Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity.

Information d'application

Indications d'application: Dot blot(ECM)|0.25-0.5 μg/mL| Western blot(DAB)|0.3-2 μg/mL| Western blot(ECM)|0.1-0.3 μg/mL| ELISA|0.025-0.05 μg/mL|

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Stock: 4 °C

Stockage commentaire: At 4°C for one year.

Date de péremption: 12 months

Référence

Tian, Shen, Chen: "Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway." dans: Oncology reports, Vol. 38, Issue 2, pp. 745-754, (2018) (PubMed).

Bian, Yang, Ma, Li, Duan, Wei, Wu, Mu, Lin, Wen, Xi: "Beneficial effects of extracts from Lucilia sericata maggots on burn wounds in rats." dans: Molecular medicine reports, Vol. 16, Issue 5, pp. 7213-7220, (2018) (PubMed).

Zhang, Fu, Tang: "The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats." dans: Molecular medicine reports, Vol. 17, Issue 3, pp. 3455-3464, (2018) (PubMed).

Zuo, Lu, Yan, Pan, Cheng: "Increased expression of hepcidin and associated upregulation of JAK/STAT3 signaling in human gastric cancer." dans: Oncology letters, Vol. 15, Issue 2, pp. 2236-2244, (2018) (PubMed).

Zhang, Tan, Yu, Wang, Chen, Han, Li, Chen, Xiao, Ambati, Cai, Yang, Nayak, Zhang, Fan: "Placenta-specific drug delivery by trophoblast-targeted nanoparticles in mice." dans: Theranostics, Vol. 8, Issue 10, pp. 2765-2781, (2018) (PubMed).

Wang, Shen, Zhang, Wang, Xu, Chen, Chen, Yang, He, Wang, Su, Cheng, Zhao, Wang: "Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5." dans: Scientific reports, Vol. 6, pp. 22875, (2017) (PubMed).

Shen, Ma, Shuai, Yang: "Effects of 1,25-dihydroxyvitamin D3 on the local bone renin-angiotensin system in a murine model of glucocorticoid-induced osteoporosis." dans: Experimental and therapeutic medicine, Vol. 13, Issue 6, pp. 3297-3304, (2017) (PubMed).

Li, Gao, Qi: "PDCD1 strengthens the sensitivity of ovarian cancer to cisplatin chemotherapy by promoting apoptosis." dans: Journal of B.U.ON. : official journal of the Balkan Union of Oncology, Vol. 22, Issue 3, pp. 746-756, (2017) (PubMed).

Zhang, Zhao, Zhao, Yan, Yang, Huang, Chen, Fu, Lv: "The Protective Effect of Salidroside on Hypoxia-Induced Corpus Cavernosum Smooth Muscle Cell Phenotypic Transformation." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3530281, (2017) (PubMed).

Xin, Sun, Kong, Li, Zheng, Yin, Cao, Jia, Li: "Intercellular adhesion molecule-1 expression in activated eosinophils is associated with mucosal remodeling in nasal polyps." dans: Molecular medicine reports, Vol. 11, Issue 5, pp. 3391-7, (2015) (PubMed).

Jin, Liu, Deng, Liao, Lin, Ning, Luo et al.: "Neuroprotective effects of activated protein C on intrauterine inflammation-induced neonatal white matter injury are associated with the downregulation of fibrinogen-like protein 2/fibroleukin ..." dans: International journal of molecular medicine, Vol. 35, Issue 5, pp. 1199-212, (2015) (PubMed).

Tang, Zhang, Yuan, Gao, Wang, Ye, Li, Gao, Miao, Wang, Jiang: "Pancreatic satellite cells derived galectin-1 increase the progression and less survival of pancreatic ductal adenocarcinoma." dans: PLoS ONE, Vol. 9, Issue 3, pp. e90476, (2015) (PubMed).

Cheng, Wan, Liu, Zeng, Xing, Wu, Chen, Zhu: "Overexpression of SATB1 is associated with biologic behavior in human renal cell carcinoma." dans: PLoS ONE, Vol. 9, Issue 5, pp. e97406, (2015) (PubMed).

Nguyen, Tsou, Calarese, Kimani, Singh, Hsieh, Liu, Lu, Wu, Garforth, Almo, Kotenko, Birge: "Overexpression of MERTK receptor tyrosine kinase in epithelial cancer cells drives efferocytosis in a gain-of-function capacity." dans: The Journal of biological chemistry, Vol. 289, Issue 37, pp. 25737-49, (2015) (PubMed).

Liu, Lan, Huang, Wang, Xu, Shi, Wu, Wu, Zhang, Chen, Wang, Yu, Zhu, Lu et al.: "Inhibition of Lon blocks cell proliferation, enhances chemosensitivity by promoting apoptosis and decreases cellular bioenergetics of bladder cancer: potential roles of Lon as a prognostic marker and ..." dans: Oncotarget, Vol. 5, Issue 22, pp. 11209-24, (2015) (PubMed).

Qu, Xia, Zhang, Pan, Zhao: "Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin." dans: Oncology reports, Vol. 33, Issue 3, pp. 1177-84, (2015) (PubMed).

Gan, Wang, Zhou, Lu, Zhu, Tang, Jian, Wu: "Molecular and functional characterization of CD59 from Nile tilapia (Oreochromis niloticus) involved in the immune response to Streptococcus agalactiae." dans: Fish & shellfish immunology, Vol. 44, Issue 1, pp. 50-9, (2015) (PubMed).

Wu, Wang, Zhang, Zhang, Zhang, Zhang: "Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats." dans: Neural regeneration research, Vol. 7, Issue 6, pp. 421-5, (2015) (PubMed).

Qian, Gong, Yang, Chen, Chen, Xu, Wu, Tang, Gao, Zeng: "Diastolic dysfunction in spontaneous type 2 diabetes rhesus monkeys: a study using echocardiography and magnetic resonance imaging." dans: BMC cardiovascular disorders, Vol. 15, pp. 59, (2015) (PubMed).

Wang, Yao, Huang, Li, Xu: "Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage." dans: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 995-1002, (2015) (PubMed).

Détail du antigène

Antigène: Streptavidin

Abstract: Streptavidin Produits

Sujet: Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity.