SUMO3 Protein (AA 1-92) (His tag)

N° du produit ABIN3073484

Détail du produit

Antigène: SUMO3 (Small Ubiquitin Related Modifier 3 (SUMO3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-92

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette SUMO3 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: MSEEKPKEGV KTENDHINLK VAGQDGSVVQ FKIKRHTPLS KLMKAYCERQ GLSMRQIRFR FDGQPINETD TPAQLEMEDE DTIDVFQQQT GG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SUMO3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SUMO3 (Small Ubiquitin Related Modifier 3 (SUMO3))

Autre désignation: SUMO3 (SUMO3 Produits)

Synonymes: SMT3A Protein, SMT3H1 Protein, SUMO-3 Protein, Smt3B Protein, 2810014B19Rik Protein, AA409334 Protein, AW121497 Protein, AW536077 Protein, D10Ertd345e Protein, Smt3h1 Protein, smt3a Protein, smt3h1 Protein, fa94a11 Protein, im:7141764 Protein, sumo2 Protein, sumo3 Protein, sumo3l Protein, wu:fa94a11 Protein, wu:fb55d09 Protein, zgc:65847 Protein, zgc:85643 Protein, fc66f10 Protein, fl11h12 Protein, wu:fc66f10 Protein, wu:fl11h12 Protein, zgc:86902 Protein, SUMO3 Protein, ATSUMO3 Protein, MCO15.12 Protein, MCO15_12 Protein, SMALL UBIQUITIN-LIKE MODIFIER 3 Protein, SUM3 Protein, SUMO 3 Protein, small ubiquitin-like modifier 3 Protein, GMP1 Protein, PIC1 Protein, SENTRIN Protein, SMT3 Protein, SMT3H3 Protein, SMTP3 Protein, SUMO-1 Protein, Smt3C Protein, Ubl1 Protein, small ubiquitin-like modifier 3 Protein, small ubiquitin-like modifier 3 L homeolog Protein, small ubiquitin-like modifier 3a Protein, small ubiquitin-like modifier 3b Protein, small ubiquitin-related modifier 2 pseudogene Protein, SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) Protein, small ubiquitin-like modifier 2 Protein, small ubiquitin-like modifier 1 Protein, SUMO3 Protein, Sumo3 Protein, sumo3.L Protein, sumo3a Protein, sumo3b Protein, LOC490297 Protein, sumo3 Protein, sumo2 Protein, Sumo1 Protein

Sujet: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 (PubMed:11451954, PubMed:18538659, PubMed:21965678). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744). {ECO:0000269|PubMed:11451954, ECO:0000269|PubMed:18538659, ECO:0000269|PubMed:21965678}.

Poids moléculaire: 11.5 kDa Including tag.

UniProt: P55854