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TOMM22 Protein (AA 2-142) (rho-1D4 tag)

Crystallography grade TOMM22 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3074708
  • Antigène Voir toutes TOMM22 Protéines
    TOMM22 (Translocase of Outer Mitochondrial Membrane 22 Homolog (TOMM22))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-142
    Origine
    • 3
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 3
    • 2
    • 2
    Cellules d'insectes
    Purification/Conjugué
    Cette TOMM22 protéine est marqué à la rho-1D4 tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    AAAVAAAGAG EPQSPDELLP KGDAEKPEEE LEEDDDEELD ETLSERLWGL TEMFPERVRS AAGATFDLSL FVAQKMYRFS RAALWIGTTS FMILVLPVVF ETEKLQMEQQ QQLQQRQILL GPNTGLSGGM PGALPSLPGK I
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TOMM22 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product TOMM22 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    TOMM22 (Translocase of Outer Mitochondrial Membrane 22 Homolog (TOMM22))
    Autre désignation
    TOMM22 (TOMM22 Produits)
    Synonymes
    cb381 Protein, sb:cb381 Protein, zgc:86742 Protein, TOMM22 Protein, 1C9-2 Protein, MST065 Protein, MSTP065 Protein, TOM22 Protein, 2310047D01 Protein, Tom22 Protein, rTOM22 Protein, translocase of outer mitochondrial membrane 22 homolog (yeast) Protein, translocase of outer mitochondrial membrane 22 Protein, tomm22 Protein, TOMM22 Protein, Tomm22 Protein
    Sujet
    Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. {ECO:0000269|PubMed:10982837}.
    Poids moléculaire
    16.6 kDa Including tag.
    UniProt
    Q9NS69
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