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Transformer 2 beta Homolog (TRA2B) (AA 2-288) protein (His tag) Protein

Crystallography grade TRA2B Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3074774
$6,086.30
Plus frais de port $45.00 et $20.00 pour carboglace
Destination: Etats-Unis
Envoi sous 30 à 35 jours ouvrables
  • Antigène Voir toutes TRA2B Protéines
    TRA2B
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-288
    Origine
    • 5
    • 1
    • 1
    Humain
    Source
    • 3
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette TRA2B protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    SDSGEQNYGE RESRSASRSG SAHGSGKSAR HTPARSRSKE DSRRSRSKSR SRSESRSRSR RSSRRHYTRS RSRSRSHRRS RSRSYSRDYR RRHSHSHSPM STRRRHVGNR ANPDPNCCLG VFGLSLYTTE RDLREVFSKY GPIADVSIVY DQQSRRSRGF AFVYFENVDD AKEAKERANG MELDGRRIRV DFSITKRPHT PTPGIYMGRP TYGSSRRRDY YDRGYDRGYD DRDYYSRSYR GGGGGGGGWR AAQDRDQIYR RRSPSPYYSR GGYRSRSRSR SYSPRRY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TRA2B Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    TRA2B
    Autre désignation
    TRA2B (TRA2B Produits)
    Synonymes
    sfrs10, SFRS10, Htra2-beta, SRFS10, TRA2-BETA, TRAN2B, 5730405G21Rik, D16Ertd266e, SIG-41, Sfrs10, Silg41, TRA2beta, Srsf10, zgc:56612, transformer 2 beta homolog (Drosophila), transformer 2 beta homolog L homeolog, transformer 2 beta homolog, tra2b, tra2b.L, TRA2B, Tra2b
    Sujet
    Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA. {ECO:0000269|PubMed:12165565, ECO:0000269|PubMed:12761049, ECO:0000269|PubMed:15009664, ECO:0000269|PubMed:9546399}.
    Poids moléculaire
    34.5 kDa Including tag.
    UniProt
    P62995
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