TRIM29 Protein (AA 1-588) (His tag)
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- Antigène Voir toutes TRIM29 Protéines
- TRIM29 (Tripartite Motif Containing 29 (TRIM29))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-588
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Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette TRIM29 protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
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MEAADASRSN GSSPEARDAR SPSGPSGSLE NGTKADGKDA KTTNGHGGEA AEGKSLGSAL KPGEGRSALF AGNEWRRPII QFVESGDDKN SNYFSMDSME GKRSPYAGLQ LGAAKKPPVT FAEKGELRKS IFSESRKPTV SIMEPGETRR NSYPRADTGL FSRSKSGSEE VLCDSCIGNK QKAVKSCLVC QASFCELHLK PHLEGAAFRD HQLLEPIRDF EARKCPVHGK TMELFCQTDQ TCICYLCMFQ EHKNHSTVTV EEAKAEKETE LSLQKEQLQL KIIEIEDEAE KWQKEKDRIK SFTTNEKAIL EQNFRDLVRD LEKQKEEVRA ALEQREQDAV DQVKVIMDAL DERAKVLHED KQTREQLHSI SDSVLFLQEF GALMSNYSLP PPLPTYHVLL EGEGLGQSLG NFKDDLLNVC MRHVEKMCKA DLSRNFIERN HMENGGDHRY VNNYTNSFGG EWSAPDTMKR YSMYLTPKGG VRTSYQPSSP GRFTKETTQK NFNNLYGTKG NYTSRVWEYS SSIQNSDNDL PVVQGSSSFS LKGYPSLMRS QSPKAQPQTW KSGKQTMLSH YRPFYVNKGN GIGSNEAP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human TRIM29 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product TRIM29 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- TRIM29 (Tripartite Motif Containing 29 (TRIM29))
- Autre désignation
- TRIM29 (TRIM29 Produits)
- Synonymes
- ATDC Protein, 1110047J21Rik Protein, 2810431N19Rik Protein, 4732461M22Rik Protein, AI119726 Protein, MGC53484 Protein, TRIM29 Protein, atdc Protein, tripartite motif containing 29 Protein, tripartite motif-containing 29 Protein, tripartite motif containing 29 L homeolog Protein, TRIM29 Protein, Trim29 Protein, trim29.L Protein, trim29 Protein
- Sujet
- It is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line.
- Poids moléculaire
- 66.8 kDa Including tag.
- UniProt
- Q14134
- Pathways
- Signalisation p53, Apoptose, Réparation de l'ADN, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
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