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TXNDC16 Protein (AA 28-825) (His tag)

Crystallography grade TXNDC16 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3074960
  • Antigène Tous les produits TXNDC16
    TXNDC16 (Thioredoxin Domain Containing 16 (TXNDC16))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 28-825
    Origine
    Humain
    Source
    Cellules d'insectes
    Purification/Conjugué
    Cette TXNDC16 protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    LPELSPQKYF STLQPGKASL AYFCQADSPR TSVFLEELNE AVRPLQDYGI SVAKVNCVKE EISRYCGKEK DLMKAYLFKG NILLREFPTD TLFDVNAIVA HVLFALLFSE VKYITNLEDL QNIENALKGK ANIIFSYVRA IGIPEHRAVM EAAFVYGTTY QFVLTTEIAL LESIGSEDVE YAHLYFFHCK LVLDLTQQCR RTLMEQPLTT LNIHLFIKTM KAPLLTEVAE DPQQVSTVHL QLGLPLVFIV SQQATYEADR RTAEWVAWRL LGKAGVLLLL RDSLEVNIPQ DANVVFKRAE EGVPVEFLVL HDVDLIISHV ENNMHIEEIQ EDEDNDMEGP DIDVQDDEVA ETVFRDRKRK LPLELTVELT EETFNATVMA SDSIVLFYAG WQAVSMAFLQ SYIDVAVKLK GTSTMLLTRI NCADWSDVCT KQNVTEFPII KMYKKGENPV SYAGMLGTED LLKFIQLNRI SYPVNITSIQ EAEEYLSGEL YKDLILYSSV SVLGLFSPTM KTAKEDFSEA GNYLKGYVIT GIYSEEDVLL LSTKYAASLP ALLLARHTEG KIESIPLAST HAQDIVQIIT DALLEMFPEI TVENLPSYFR LQKPLLILFS DGTVNPQYKK AILTLVKQKY LDSFTPCWLN LKNTPVGRGI LRAYFDPLPP LPLLVLVNLH SGGQVFAFPS DQAIIEENLV LWLKKLEAGL ENHITILPAQ EWKPPLPAYD FLSMIDAATS QRGTRKVPKC MKETDVQEND KEQHEDKSAV RKEPIETLRI KHWNRSNWFK EAEKSFRRDK ELGCSKVN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TXNDC16 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    TXNDC16 (Thioredoxin Domain Containing 16 (TXNDC16))
    Autre désignation
    TXNDC16 (TXNDC16 Produits)
    Synonymes
    ERp90 Protein, KIAA1344 Protein, 5730420B22Rik Protein, C77647 Protein, RGD1306755 Protein, thioredoxin domain containing 16 Protein, TXNDC16 Protein, Txndc16 Protein
    Poids moléculaire
    91.4 kDa Including tag.
    UniProt
    Q9P2K2
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