ATP6V0A1 Protein (AA 1-388) (His tag)

N° du produit ABIN3075367

Détail du produit

Antigène: ATP6V0A1 (ATPase, H+ Transporting, Lysosomal V0 Subunit A1 (ATP6V0A1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-388

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ATP6V0A1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: MGELFRSEEM TLAQLFLQSE AAYCCVSELG ELGKVQFRDL NPDVNVFQRK FVNEVRRCEE MDRKLRFVEK EIRKANIPIM DTGENPEVPF PRDMIDLEAN FEKIENELKE INTNQEALKR NFLELTELKF ILRKTQQFFD EMADPDLLEE SSSLLEPSEM GRGTPLRLGF VAGVINRERI PTFERMLWRV CRGNVFLRQA EIENPLEDPV TGDYVHKSVF IIFFQGDQLK NRVKKICEGF RASLYPCPET PQERKEMASG VNTRIDDLQM VLNQTEDHRQ RVLQAAAKNI RVWFIKVRKM KAIYHTLNLC NIDVTQKCLI AEVWCPVTDL DSIQFALRRG TEHSGSTVPS ILNRMQTNQT PPTYNKTNKF TYGFQNIVDA YGIGTYRE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ATP6V0A1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ATP6V0A1 (ATPase, H+ Transporting, Lysosomal V0 Subunit A1 (ATP6V0A1))

Autre désignation: ATP6V0A1 (ATP6V0A1 Produits)

Synonymes: ATP6N1 Protein, ATP6N1A Protein, Stv1 Protein, VPP1 Protein, Vph1 Protein, a1 Protein, AA959968 Protein, ATP6a1 Protein, Atp6n1 Protein, Atp6n1a Protein, Atpv0a1 Protein, Vpp-1 Protein, Vpp1 Protein, A1 Protein, wu:fj37e01 Protein, zgc:112214 Protein, atp6v0a1 Protein, wu:fc25b09 Protein, zgc:76965 Protein, ATPase H+ transporting V0 subunit a1 Protein, ATPase, H+ transporting, lysosomal V0 subunit A1 Protein, si:ch73-173p19.2 Protein, ATPase, H+ transporting, lysosomal V0 subunit a1 L homeolog Protein, ATPase, H+ transporting, lysosomal V0 subunit a1b Protein, ATPase, H+ transporting, lysosomal V0 subunit a1a Protein, ATP6V0A1 Protein, Atp6v0a1 Protein, si:ch73-173p19.2 Protein, atp6v0a1.L Protein, atp6v0a1b Protein, atp6v0a1a Protein

Sujet: Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity). {ECO:0000250}.

Poids moléculaire: 46.1 kDa Including tag.

UniProt: Q93050

Pathways: Transition Metal Ion Homeostasis, Proton Transport