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Vacuolar Protein-Sorting-Associated Protein 25 (VPS25) (AA 1-176) protein (His tag) Protein

Crystallography grade VPS25 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3075393
$6,086.30
Plus frais de port $45.00 et $20.00 pour carboglace
Destination: Etats-Unis
Envoi sous 30 à 35 jours ouvrables
  • Antigène Voir toutes VPS25 Protéines
    VPS25
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-176
    Origine
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 5
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette VPS25 protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TNGEDTEDEE FHGLDEATLL RALQALQQEH KAEIITVSDG RGVKFF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human VPS25 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    VPS25
    Autre désignation
    VPS25 (VPS25 Produits)
    Synonymes
    VPS25, CG14750, Dmel\\CG14750, ESCRT-I, ESCRT-II, dVps25, l(2)44Db, l(2)k08904, vps25, GB16268, derp9, eap20, fap20, DDBDRAFT_0189483, DDBDRAFT_0234026, DDB_0189483, DDB_0234026, 1110020N13Rik, AW107467, D11Wsu68e, im:6898134, zgc:162560, DERP9, EAP20, FAP20, vacuolar protein sorting 25 homolog, vacuolar protein sorting 25 homolog (S. cerevisiae) pseudogene, vacuolar protein sorting-associated protein 25, WNK lysine deficient protein kinase 4, Vacuolar protein sorting 25, vacuolar protein-sorting-associated protein 25, vacuolar protein sorting 25 homolog L homeolog, vacuolar protein sorting 25, vacuolar protein sorting 25 homolog (S. cerevisiae), E2F/DP family winged-helix DNA-binding domain-containing protein, VPS25, LOC459431, CpipJ_CPIJ010292, WNK4, Vps25, LOC412381, vps25.L, vps25
    Sujet
    Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. {ECO:0000269|PubMed:18723511}.
    Poids moléculaire
    21.7 kDa Including tag.
    UniProt
    Q9BRG1
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