WDR43 Protein (AA 1-677) (Strep Tag)

N° du produit ABIN3075605

Détail du produit

Antigène: WDR43 (WD Repeat Domain 43 (WDR43))

Type de proteíne: Recombinant

Attributs du protein: AA 1-677

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette WDR43 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Séquence: MAAGGGGSCD PLAPAGVPCA FSPHSQAYFA LASTDGHLRV WETANNRLHQ EYVPSAHLSG TCTCLAWAPA RLQAKESPQR KKRKSEAVGM SNQTDLLALG TAVGSILLYS TVKGELHSKL ISGGHDNRVN CIQWHQDSGC LYSCSDDKHI VEWNVQTCKV KCKWKGDNSS VSSLCISPDG KMLLSAGRTI KLWVLETKEV YRHFTGHATP VSSLMFTTIR PPNESQPFDG ITGLYFLSGA VHDRLLNVWQ VRSENKEKSA VMSFTVTDEP VYIDLTLSEN KEEPVKLAVV CRDGQVHLFE HILNGYCKKP LTSNCTIQIA TPGKGKKSTP KPIPILAAGF CSDKMSLLLV YGSWFQPTIE RVALNSREPH MCLVRDISNC WAPKVETAIT KVRTPVMNSE AKVLVPGIPG HHAAIKPAPP QTEQVESKRK SGGNEVSIEE RLGAMDIDTH KKGKEDLQTN SFPVLLTQGL ESNDFEMLNK VLQTRNVNLI KKTVLRMPLH TIIPLLQELT KRLQGHPNSA VLMVQWLKCV LTVHASYLST LPDLVPQLGT LYQLMESRVK TFQKLSHLHG KLILLITQVT ASEKTKGATS PGQKAKLVYE EESSEEESDD EIADKDSEDN WDEDEEESES EKDEDVEEED EDAEGKDEEN GEDRDTASEK ELNGDSDLDP ENESEEE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: WDR43 (WD Repeat Domain 43 (WDR43))

Autre désignation: WDR43 (WDR43 Produits)

Synonymes: NET12 Protein, UTP5 Protein, 2610318G08Rik Protein, AU020887 Protein, AV024208 Protein, Wrd43 Protein, mKIAA0007 Protein, WD repeat domain 43 Protein, WDR43 Protein, Wdr43 Protein

Sujet: WD repeat-containing protein 43 (U3 small nucleolar RNA-associated protein 5 homolog),FUNCTION: Ribosome biogenesis factor that coordinates hyperactive transcription and ribogenesis (PubMed:17699751). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I (PubMed:17699751, PubMed:34516797). Essential for stem cell pluripotency and embryonic development. In the nucleoplasm, recruited by promoter-associated/nascent transcripts and transcription to active promoters where it facilitates releases of elongation factor P-TEFb and paused RNA polymerase II to allow transcription elongation and maintain high-level expression of its targets genes (By similarity). {ECO:0000250|UniProtKB:Q6ZQL4, ECO:0000269|PubMed:17699751, ECO:0000269|PubMed:34516797}.

Poids moléculaire: 74.9 kDa

UniProt: Q15061