Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

SMU1 Protein (AA 2-513) (His tag)

Crystallography grade SMU1 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3077293
  • Antigène Voir toutes SMU1 Protéines
    SMU1 (Smu-1 Suppressor of Mec-8 and Unc-52 Homolog (SMU1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-513
    Origine
    • 3
    • 2
    • 1
    Humain
    Source
    • 3
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette SMU1 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    SIEIESSDVI RLIMQYLKEN SLHRALATLQ EETTVSLNTV DSIESFVADI NSGHWDTVLQ AIQSLKLPDK TLIDLYEQVV LELIELRELG AARSLLRQTD PMIMLKQTQP ERYIHLENLL ARSYFDPREA YPDGSSKEKR RAAIAQALAG EVSVVPPSRL MALLGQALKW QQHQGLLPPG MTIDLFRGKA AVKDVEEEKF PTQLSRHIKF GQKSHVECAR FSPDGQYLVT GSVDGFIEVW NFTTGKIRKD LKYQAQDNFM MMDDAVLCMC FSRDTEMLAT GAQDGKIKVW KIQSGQCLRR FERAHSKGVT CLSFSKDSSQ ILSASFDQTI RIHGLKSGKT LKEFRGHSSF VNEATFTQDG HYIISASSDG TVKIWNMKTT ECSNTFKSLG STAGTDITVN SVILLPKNPE HFVVCNRSNT VVIMNMQGQI VRSFSSGKRE GGDFVCCALS PRGEWIYCVG EDFVLYCFST VTGKLERTLT VHEKDVIGIA HHPHQNLIAT YSEDGLLKLW KP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SMU1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product SMU1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    SMU1 (Smu-1 Suppressor of Mec-8 and Unc-52 Homolog (SMU1))
    Autre désignation
    SMU1 (SMU1 Produits)
    Synonymes
    SMU1 Protein, BWD Protein, RP11-54K16.3 Protein, SMU-1 Protein, fSAP57 Protein, 2600001O03Rik Protein, 2610203K23Rik Protein, AB044414 Protein, AI845086 Protein, AW556129 Protein, Bwd Protein, Smu-1 Protein, zgc:56147 Protein, SMU1, DNA replication regulator and spliceosomal factor Protein, WD40 repeat-containing protein SMU1 Protein, smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans) Protein, DNA replication regulator and spliceosomal factor Protein, SMU1, DNA replication regulator and spliceosomal factor a Protein, SMU1 Protein, LOC100180093 Protein, Smu1 Protein, smu1a Protein
    Poids moléculaire
    58.4 kDa Including tag.
    UniProt
    Q2TAY7
Vous êtes ici:
Support technique