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CHORDC1 Protein (AA 2-332) (His tag)

Crystallography grade CHORDC1 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3077796
  • Antigène Voir toutes CHORDC1 Protéines
    CHORDC1 (Cysteine and Histidine-Rich Domain (CHORD)-Containing 1 (CHORDC1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-332
    Origine
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 6
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CHORDC1 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    ALLCYNRGCG QRFDPETNSD DACTYHPGVP VFHDALKGWS CCKRRTTDFS DFLSIVGCTK GRHNSEKPPE PVKPEVKTTE KKELCELKPK FQEHIIQAPK PVEAIKRPSP DEPMTNLELK ISASLKQALD KLKLSSGNEE NKKEEDNDEI KIGTSCKNGG CSKTYQGLES LEEVCVYHSG VPIFHEGMKY WSCCRRKTSD FNTFLAQEGC TKGKHMWTKK DAGKKVVPCR HDWHQTGGEV TISVYAKNSL PELSRVEANS TLLNVHIVFE GEKEFDQNVK LWGVIDVKRS YVTMTATKIE ITMRKAEPMQ WASLELPAAK KQEKQKDATT D
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CHORDC1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CHORDC1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CHORDC1 (Cysteine and Histidine-Rich Domain (CHORD)-Containing 1 (CHORDC1))
    Autre désignation
    CHORDC1 (CHORDC1 Produits)
    Synonymes
    CHP1 Protein, CHP-1 Protein, MGC89160 Protein, NV16801 Protein, Chp-1 Protein, Morgana Protein, 1110001O09Rik Protein, AA409036 Protein, morgana Protein, zgc:63894 Protein, cysteine and histidine rich domain containing 1 Protein, cysteine and histidine-rich domain-containing protein Protein, cysteine and histidine rich domain containing 1 S homeolog Protein, cysteine and histidine-rich domain (CHORD)-containing 1 Protein, cysteine and histidine-rich domain (CHORD)-containing, zinc-binding protein 1 Protein, cysteine and histidine-rich domain (CHORD) containing 1b Protein, CHORDC1 Protein, Chordc1 Protein, LOC412067 Protein, chordc1.S Protein, chordc1 Protein, chordc1b Protein
    Sujet
    Regulates centrosome duplication, probably by inhibiting the kinase activity of ROCK2. Proposed to act as co-chaperone for HSP90. May play a role in the regulation of NOD1 via a HSP90 chaperone complex. In vitro, has intrinsic chaperone activity. This function may be achieved by inhibiting association of ROCK2 with NPM1. Involved in stress response. Prevents tumorigenesis. {ECO:0000269|PubMed:20230755}.
    Poids moléculaire
    38.3 kDa Including tag.
    UniProt
    Q9UHD1
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