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C4B Protein (AA 957-1336) (His tag)

Crystallography grade C4b Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3078027
  • Antigène Voir toutes C4B (C4b) Protéines
    C4B (C4b) (Complement Component C4b (C4b))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 957-1336
    Origine
    • 13
    • 6
    • 2
    Humain
    Source
    • 9
    • 7
    • 3
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette C4B protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    TLEIPGNSDP NMIPDGDFNS YVRVTASDPL DTLGSEGALS PGGVASLLRL PRGCGEQTMI YLAPTLAASR YLDKTEQWST LPPETKDHAV DLIQKGYMRI QQFRKADGSY AAWLSRGSST WLTAFVLKVL SLAQEQVGGS PEKLQETSNW LLSQQQADGS FQDLSPVIHR SMQGGLVGND ETVALTAFVT IALHHGLAVF QDEGAEPLKQ RVEASISKAS SFLGEKASAG LLGAHAAAIT AYALTLTKAP ADLRGVAHNN LMAMAQETGD NLYWGSVTGS QSNAVSPTPA PRNPSDPMPQ APALWIETTA YALLHLLLHE GKAEMADQAA AWLTRQGSFQ GGFRSTQDTV IALDALSAYW IASHTTEERG LNVTLSSTGR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human C4B, C4B_2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product C4b Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    C4B (C4b) (Complement Component C4b (C4b))
    Autre désignation
    C4B, C4B_2 (C4b Produits)
    Synonymes
    C4B1 Protein, C4B12 Protein, C4B2 Protein, C4B3 Protein, C4B5 Protein, C4BD Protein, C4B_2 Protein, C4F Protein, CH Protein, CO4 Protein, CPAMD3 Protein, C4 Protein, C4A2 Protein, C4A3 Protein, C4A4 Protein, C4A6 Protein, C4AD Protein, C4S Protein, CPAMD2 Protein, RG Protein, wu:fi14a03 Protein, C4B Protein, DKFZP469I0332 Protein, Slp Protein, Ss Protein, C4-2 Protein, C4l Protein, complement C4B (Chido blood group) Protein, complement C4A (Rodgers blood group) Protein, complement component 4 Protein, complement C4-B Protein, complement component 4B (Chido blood group) Protein, complement component 4A (Rodgers blood group) Protein, C4B Protein, C4A Protein, c4 Protein, LOC462577 Protein, C4a Protein, C4b Protein
    Sujet
    Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens., Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    Poids moléculaire
    41.5 kDa Including tag.
    UniProt
    P0C0L5
    Pathways
    Système du Complément
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