Desmocollin 3 Protein (DSC3) (AA 712-896) (His tag)

N° du produit ABIN3079072

Détail du produit

Antigène: Desmocollin 3 (DSC3)

Type de proteíne: Recombinant

Attributs du protein: AA 712-896

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette Desmocollin 3 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: MCGVFGATKG KRFPEDLAQQ NLIISNTEAP GDDRVCSANG FMTQTTNNSS QGFCGTMGSG M KNGGQETIEM MKGGNQTLES CRGAGHHHTL DSCRGGHTEV DNCRYTYSEW HSFTQPRLGE KLHRCNQNED RMPSQDYVLT YNYEGRGSPA GSVGCCSEKQ EEDGLDFLNN LEPKFITLAE ACTKRHHHHH H
Sequence includes C-terminal His-tag

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In solution (20 mM Hepes, pH 7.4, 100 mM NaCl,)

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Référence

Chernyavsky, Amber, Agnoletti, Wang, Grando: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." dans: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).

Détail du antigène

Antigène: Desmocollin 3 (DSC3)

Autre désignation: DSC3 (DSC3 Produits)

Sujet: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.

Poids moléculaire: 21.3 kDa Including tag

UniProt: Q14574