Desmoglein 1 Protein (DSG1) (AA 50-548) (His tag)
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- Antigène Voir toutes Desmoglein 1 (DSG1) Protéines
- Desmoglein 1 (DSG1)
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 50-548
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Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette Desmoglein 1 protéine est marqué à la His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Séquence
- MEWIKFAAAC REGEDNSKRN PIAKIHSDCA ANQQVTYRIS GVGIDQPPYG IFVINQKTGE INITSIVDRE VTPFFIIYCR ALNSMGQDLE RPLELRVRVL DINDNPPVFS MATFAGQIEE NSNANTLVMI LNATDADEPN NLNSKIAFKI IRQEPSDSPM FIINRNTGEI RTMNNFLDRE QYGQYALAVR GSDRDGGADG MSAECECNIK ILDVNDNIPY MEQSSYTIEI QENTLNSNLL EIRVIDLDEE FSANWMAVIF FISGNEGNWF EIEMNERTNV GILKVVKPLD YEAMQSLQLS IGVRNKAEFH HSIMSQYKLK ASAISVTVLN VIEGPVFRPG SKTYVVTGNM GSNDKVGDFV ATDLDTGRPS TTVRYVMGNN PADLLAVDSR TGKLTLKNKV TKEQYNMLGG KYQGTILSID DNLQRTCTGT ININIQSFGN DDRTNTEPNT KITTNTGRQE STSSTNYDTS TTSTDSSQVY SSEPGNGAKD LLSDNVHFGP HHHHHH
- Specificité
- C-terminal His-tag
- Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human DSG1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount..
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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C-terminal His-tag
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 20 mM Hepes, pH 7.5; 150 mM NaCl; 2% Lauroylsarcosin; 1mM EDTA; 1mM DTT
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- Desmoglein 1 (DSG1)
- Autre désignation
- DSG1 (DSG1 Produits)
- Synonymes
- CDHF4 Protein, DG1 Protein, DSG Protein, PPKS1 Protein, SPPK1 Protein, DSG-1 Protein, DSG1 Protein, Dsg1b Protein, Dsg1c Protein, desmoglein 1 Protein, desmoglein 4 Protein, DSG1 Protein, DSG4 Protein, Dsg1 Protein
- Sujet
- Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
- Poids moléculaire
- 56.3 kDa Including tag.
- UniProt
- Q02413
- Pathways
- Cell-Cell Junction Organization
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