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EIF3K Protein (AA 2-218) (His tag)

Crystallography grade EIF3K Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
N° du produit ABIN3079326
  • Antigène Voir toutes EIF3K Protéines
    EIF3K (Eukaryotic Translation Initiation Factor 3 Subunit K (EIF3K))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-218
    Origine
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 8
    • 1
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette EIF3K protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Séquence
    AMFEQMRANV GKLLKGIDRY NPENLATLER YVETQAKENA YDLEANLAVL KLYQFNPAFF QTTVTAQILL KALTNLPHTD FTLCKCMIDQ AHQEERPIRQ ILYLGDLLET CHFQAFWQAL DENMDLLEGI TGFEDSVRKF ICHVVGITYQ HIDRWLLAEM LGDLSDSQLK VWMSKYGWSA DESGQIFICS QEESIKPKNI VEKIDFDSVS SIMASSQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human EIF3K Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product EIF3K Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    EIF3K (Eukaryotic Translation Initiation Factor 3 Subunit K (EIF3K))
    Autre désignation
    EIF3K (EIF3K Produits)
    Synonymes
    eif3s12 Protein, EIF3S12 Protein, ACYPI000023 Protein, eIF3k Protein, EIF3-p28 Protein, HSPC029 Protein, M9 Protein, MSTP001 Protein, PLAC-24 Protein, PLAC24 Protein, PRO1474 Protein, PTD001 Protein, 1200009C21Rik Protein, Eif3s12 Protein, zgc:110726 Protein, eukaryotic translation initiation factor 3 subunit K L homeolog Protein, eukaryotic translation initiation factor 3 subunit K Protein, eukaryotic translation initiation factor 3, subunit K Protein, eukaryotic translation initiation factor 3 subunit k Protein, Eukaryotic translation initiation factor 3 subunit K Protein, eif3k.L Protein, EIF3K Protein, eif3k Protein, ANI_1_1610144 Protein, AOR_1_2266174 Protein, Tsp_00068 Protein, Tsp_13184 Protein, Eif3k Protein, LOC733122 Protein, eif-3.K Protein
    Sujet
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    Poids moléculaire
    25.9 kDa Including tag.
    UniProt
    Q9UBQ5
    Pathways
    Ribonucleoprotein Complex Subunit Organization
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