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LSS Protein (AA 2-732) (His tag)

Crystallography grade LSS Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3079492
  • Antigène Voir toutes LSS Protéines
    LSS (Lanosterol Synthase (2,3-Oxidosqualene-Lanosterol Cyclase) (LSS))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-732
    Origine
    • 4
    • 3
    • 1
    Humain
    Source
    • 4
    • 1
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette LSS protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    TEGTCLRRRG GPYKTEPATD LGRWRLNCER GRQTWTYLQD ERAGREQTGL EAYALGLDTK NYFKDLPKAH TAFEGALNGM TFYVGLQAED GHWTGDYGGP LFLLPGLLIT CHVARIPLPA GYREEIVRYL RSVQLPDGGW GLHIEDKSTV FGTALNYVSL RILGVGPDDP DLVRARNILH KKGGAVAIPS WGKFWLAVLN VYSWEGLNTL FPEMWLFPDW APAHPSTLWC HCRQVYLPMS YCYAVRLSAA EDPLVQSLRQ ELYVEDFASI DWLAQRNNVA PDELYTPHSW LLRVVYALLN LYEHHHSAHL RQRAVQKLYE HIVADDRFTK SISIGPISKT INMLVRWYVD GPASTAFQEH VSRIPDYLWM GLDGMKMQGT NGSQIWDTAF AIQALLEAGG HHRPEFSSCL QKAHEFLRLS QVPDNPPDYQ KYYRQMRKGG FSFSTLDCGW IVSDCTAEAL KAVLLLQEKC PHVTEHIPRE RLCDAVAVLL NMRNPDGGFA TYETKRGGHL LELLNPSEVF GDIMIDYTYV ECTSAVMQAL KYFHKRFPEH RAAEIRETLT QGLEFCRRQQ RADGSWEGSW GVCFTYGTWF GLEAFACMGQ TYRDGTACAE VSRACDFLLS RQMADGGWGE DFESCEERRY LQSAQSQIHN TCWAMMGLMA VRHPDIEAQE RGVRCLLEKQ LPNGDWPQEN IAGVFNKSCA ISYTSYRNIF PIWALGRFSQ LYPERALAGH P
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human LSS Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product LSS Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    LSS (Lanosterol Synthase (2,3-Oxidosqualene-Lanosterol Cyclase) (LSS))
    Autre désignation
    LSS (LSS Produits)
    Synonymes
    OSC Protein, 2810025N20Rik Protein, BC029082 Protein, D10Ertd116e Protein, Osc Protein, xlss Protein, Tb07.27E10.490 Protein, NCU01119.1 Protein, lanosterol synthase Protein, lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) Protein, putative lanosterol synthase Protein, Lanosterol synthase, putative Protein, LSS Protein, Lss Protein, lss Protein, CND02520 Protein, Tc00.1047053506825.170 Protein, Tc00.1047053508175.70 Protein, Tb927.7.5230 Protein, NCU01119 Protein, LMJF_06_0650 Protein, CGB_D5080W Protein, LOC100636608 Protein
    Sujet
    Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. Through the production of lanosterol may regulate lens protein aggregation and increase transparency. {ECO:0000269|PubMed:26200341, ECO:0000269|PubMed:7639730}.
    Poids moléculaire
    84.1 kDa Including tag.
    UniProt
    P48449
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