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CES1 Protein (AA 18-567) (His tag)

Crystallography grade CES1 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3079623
  • Antigène Voir toutes CES1 Protéines
    CES1 (Carboxylesterase 1 (CES1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 18-567
    Origine
    • 9
    • 3
    • 1
    Humain
    Source
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CES1 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    GHPSSPPVVD TVHGKVLGKF VSLEGFAQPV AIFLGIPFAK PPLGPLRFTP PQPAEPWSFV KNATSYPPMC TQDPKAGQLL SELFTNRKEN IPLKLSEDCL YLNIYTPADL TKKNRLPVMV WIHGGGLMVG AASTYDGLAL AAHENVVVVT IQYRLGIWGF FSTGDEHSRG NWGHLDQVAA LRWVQDNIAS FGGNPGSVTI FGESAGGESV SVLVLSPLAK NLFHRAISES GVALTSVLVK KGDVKPLAEQ IAITAGCKTT TSAVMVHCLR QKTEEELLET TLKMKFLSLD LQGDPRESQP LLGTVIDGML LLKTPEELQA ERNFHTVPYM VGINKQEFGW LIPMQLMSYP LSEGQLDQKT AMSLLWKSYP LVCIAKELIP EATEKYLGGT DDTVKKKDLF LDLIADVMFG VPSVIVARNH RDAGAPTYMY EFQYRPSFSS DMKPKTVIGD HGDELFSVFG APFLKEGASE EEIRLSKMVM KFWANFARNG NPNGEGLPHW PEYNQKEGYL QIGANTQAAQ KLKDKEVAFW TNLFAKKAVE KPPQTEHIEL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CES1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CES1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CES1 (Carboxylesterase 1 (CES1))
    Autre désignation
    CES1 (CES1 Produits)
    Synonymes
    ACAT Protein, CE-1 Protein, CEH Protein, CES2 Protein, HMSE Protein, HMSE1 Protein, PCE-1 Protein, REH Protein, SES1 Protein, TGH Protein, hCE-1 Protein, CESDD1 Protein, CES1 Protein, CES-K1 Protein, APLE Protein, PMPMEase Protein, CES Protein, carboxylesterase 1 Protein, liver carboxylesterase 1 Protein, liver carboxylesterase Protein, carboxylesterase 1 (monocyte/macrophage serine esterase 1) Protein, CES1 Protein, CpipJ_CPIJ013026 Protein, CpipJ_CPIJ016339 Protein, LOC100009551 Protein, LOC454097 Protein, LOC699486 Protein, LOC100050915 Protein
    Sujet
    Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. {ECO:0000269|PubMed:7980644, ECO:0000269|PubMed:9169443}.
    Poids moléculaire
    61.6 kDa Including tag.
    UniProt
    P23141
    Pathways
    Monocarboxylic Acid Catabolic Process
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