GABPB2 Protein (AA 1-448) (His tag)
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- Antigène Voir toutes GABPB2 Protéines
- GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-448
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette GABPB2 protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
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MSLVDLGKRL LEAARKGQDD EVRTLMANGA PFTTDWLGTS PLHLAAQYGH YSTAEVLLRA GVSRDARTKV DRTPLHMAAA DGHAHIVELL VRNGADVNAK DMLKMTALHW ATERHHRDVV ELLIKYGADV HAFSKFDKSA FDIALEKNNA EILVILQEAM QNQVNVNPER ANPVTDPVSM AAPFIFTSGE VVNLASLISS TNTKTTSGDP HASTVQFSNS TTSVLATLAA LAEASVPLSN SHRATANTEE IIEGNSVDSS IQQVMGSGGQ RVITIVTDGV PLGNIQTSIP TGGIGQPFIV TVQDGQQVLT VPAGKVAEET VIKEEEEEKL PLTKKPRIGE KTNSVEESKE GNERELLQQQ LQEANRRAQE YRHQLLKKEQ EAEQYRLKLE AIARQQPNGV DFTMVEEVAE VDAVVVTEGE LEERETKVTG SAGTTEPHTR VSMATVSS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human GABPB2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product GABPB2 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))
- Autre désignation
- GABPB2 (GABPB2 Produits)
- Synonymes
- GABP2 Protein, GABPB1 Protein, 1810015F01Rik Protein, 5830427M07 Protein, 9430006E19Rik Protein, A430024B14Rik Protein, AV050852 Protein, Gabpb2-1 Protein, RGD1565234 Protein, gabpb2 Protein, wu:fi15e02 Protein, wu:fi17h09 Protein, zgc:55805 Protein, ankhd1 Protein, GABPB2 Protein, GABPAP Protein, im:7142141 Protein, si:dkey-77n11.3 Protein, zgc:154003 Protein, GA binding protein transcription factor beta subunit 2 Protein, GA repeat binding protein, beta 2 Protein, GA binding protein transcription factor, beta subunit 2 Protein, GA binding protein transcription factor, beta subunit 2a Protein, GA binding protein transcription factor beta subunit 2 S homeolog Protein, GA binding protein transcription factor beta subunit 1 Protein, GA binding protein transcription factor, beta subunit 2b Protein, GABPB2 Protein, Gabpb2 Protein, gabpb2a Protein, gabpb2.S Protein, GABPB1 Protein, gabpb2b Protein
- Sujet
- May function as transcription factor capable of interacting with purine rich repeats (GA repeats). {ECO:0000250}.
- Poids moléculaire
- 49.6 kDa Including tag.
- UniProt
- Q8TAK5
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