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G6PC2 Protein (AA 1-355) (rho-1D4 tag)

Crystallography grade G6PC2 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, Crys, ELISA, SDS
N° du produit ABIN3080375
  • Antigène Tous les produits G6PC2
    G6PC2 (Glucose-6-Phosphatase, Catalytic, 2 (G6PC2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-355
    Origine
    • 2
    • 1
    Humain
    Source
    • 2
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette G6PC2 protéine est marqué à la rho-1D4 tag.
    Application
    Western Blotting (WB), Crystallization (Crys), ELISA, SDS-PAGE (SDS)
    Séquence
    MDFLHRNGVL IIQHLQKDYR AYYTFLNFMS NVGDPRNIFF IYFPLCFQFN QTVGTKMIWV AVIGDWLNLI FKWILFGHRP YWWVQETQIY PNHSSPCLEQ FPTTCETGPG SPSGHAMGAS CVWYVMVTAA LSHTVCGMDK FSITLHRLTW SFLWSVFWLI QISVCISRVF IATHFPHQVI LGVIGGMLVA EAFEHTPGIQ TASLGTYLKT NLFLFLFAVG FYLLLRVLNI DLLWSVPIAK KWCANPDWIH IDTTPFAGLV RNLGVLFGLG FAINSEMFLL SCRGGNNYTL SFRLLCALTS LTILQLYHFL QIPTHEEHLF YVLSFCKSAS IPLTVVAFIP YSVHMLMKQS GKKSQGSSGTETSQV APA
    Sequence including C-terminal rho1D4 tag.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human G6PC2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    • Tag Location: C-terminal rho1D4 tag.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    The protein is purified in three steps from baculovirus infected SF9 insect cells. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot. The best performing detergents - Fos-Choline 14 and DDM - are used for solubilization and G6PC2 is purified via the C-terminal rho1D4 tag. Eluate fractions are analyzed by Western blot. Protein containing fractions of the best purification are subjected to a second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    G6PC2 (Glucose-6-Phosphatase, Catalytic, 2 (G6PC2))
    Autre désignation
    G6PC2 (G6PC2 Produits)
    Synonymes
    IGRP Protein, G6pc-rs Protein, Igrp Protein, g6pc2 Protein, igrp Protein, glucose-6-phosphatase catalytic subunit 2 Protein, glucose-6-phosphatase, catalytic, 2 Protein, glucose-6-phosphatase, catalytic, 2 S homeolog Protein, G6PC2 Protein, G6pc2 Protein, g6pc2.S Protein
    Sujet
    May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis (By similarity). {ECO:0000250}.
    Poids moléculaire
    41.8 kDa Including tag.
    UniProt
    Q9NQR9
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