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GATSL3 Protein (AA 1-329) (His tag)

Crystallography grade GATSL3 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3080584
  • Antigène Tous les produits GATSL3
    GATSL3 (GATS Protein-Like 3 (GATSL3))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-329
    Origine
    • 2
    • 1
    • 1
    • 1
    Humain
    Source
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette GATSL3 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MELHILEHRV RVLSVARPGL WLYTHPLIKL LFLPRRSRCK FFSLTETPED YTLMVDEEGF KELPPSEFLQ VAEATWLVLN VSSHSGAAVQ AAGVTKIARS VIAPLAEHHV SVLMLSTYQT DFILVREQDL SVVIHTLAQE FDIYREVGGE PVPVTRDDSS NGFPRTQHGP SPTVHPIQSP QNRFCVLTLD PETLPAIATT LIDVLFYSHS TPKEAASSSP EPSSITFFAF SLIEGYISIV MDAETQKKFP SDLLLTSSSG ELWRMVRIGG QPLGFDECGI VAQIAGPLAA ADISAYYIST FNFDHALVPE DGIGSVIEVL QRRQEGLAS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human GATSL3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    GATSL3 (GATS Protein-Like 3 (GATSL3))
    Autre désignation
    GATSL3 (GATSL3 Produits)
    Synonymes
    2410008K03Rik Protein, RGD1304774 Protein, cytosolic arginine sensor for mTORC1 subunit 1 Protein, GATS protein-like 3 Protein, CASTOR1 Protein, Gatsl3 Protein, Castor1 Protein
    Sujet
    Functions as an amino acid sensor that negatively regulates the TORC1 signaling pathway in absence of arginine. As a homooligomer or a heterooligomer with GATSL2, directly binds and inhibits the GATOR2 complex thereby preventing TORC1 signaling. When available, arginine binds GATSL3 and disrupts the interaction of GATSL3-containing oligomers with GATOR2 thereby activating the TORC1 signaling pathway. {ECO:0000269|PubMed:26972053}.
    Poids moléculaire
    37.2 kDa Including tag.
    UniProt
    Q8WTX7
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