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GABRD Protein (AA 17-248) (His tag)

Crystallography grade GABRD Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
N° du produit ABIN3080671
  • Antigène Voir toutes GABRD Protéines
    GABRD (GABA A Receptor delta (GABRD))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 17-248
    Origine
    • 3
    • 2
    Humain
    Source
    • 3
    • 2
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette GABRD protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Séquence
    QQLRGTRAMN DIGDYVGSNL EISWLPNLDG LIAGYARNFR PGIGGPPVNV ALALEVASID HISEANMEYT MTVFLHQSWR DSRLSYNHTN ETLGLDSRFV DKLWLPDTFI VNAKSAWFHD VTVENKLIRL QPDGVILYSI RITSTVACDM DLAKYPMDEQ ECMLDLESYG YSSEDIVYYW SESQEHIHGL DKLQLAQFTI TSYRFTTELM NFKSAGQFPR LSLHFHLRRN RG
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human GABRD Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    GABRD (GABA A Receptor delta (GABRD))
    Autre désignation
    GABRD (GABRD Produits)
    Synonymes
    GABRD Protein, EIG10 Protein, EJM7 Protein, GEFSP5 Protein, GABAA-RD Protein, AI853201 Protein, gamma-aminobutyric acid type A receptor delta subunit Protein, gamma-aminobutyric acid (GABA) A receptor, subunit delta Protein, GABRD Protein, Gabrd Protein
    Sujet
    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
    Poids moléculaire
    27.6 kDa Including tag.
    UniProt
    O14764
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