GRSF1 Protein (AA 118-480) (His tag)
-
- Antigène Voir toutes GRSF1 Protéines
- GRSF1 (G-Rich RNA Sequence Binding Factor 1 (GRSF1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 118-480
-
Origine
- Humain
-
Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette GRSF1 protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
-
SQESKTTYLE DLPPPPEYEL APSKLEEEVD DVFLIRAQGL PWSCTMEDVL NFFSDCRIRN GENGIHFLLN RDGKRRGDAL IEMESEQDVQ KALEKHRMYM GQRYVEVYEI NNEDVDALMK SLQVKSSPVV NDGVVRLRGL PYSCNEKDIV DFFAGLNIVD ITFVMDYRGR RKTGEAYVQF EEPEMANQAL LKHREEIGNR YIEIFPSRRN EVRTHVGSYK GKKIASFPTA KYITEPEMVF EEHEVNEDIQ PMTAFESEKE IELPKEVPEK LPEAADFGTT SSLHFVHMRG LPFQANAQDI INFFAPLKPV RITMEYSSSG KATGEADVHF ETHEDAVAAM LKDRSHVHHR YIELFLNSCP KGK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human GRSF1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product GRSF1 Protéine
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- GRSF1 (G-Rich RNA Sequence Binding Factor 1 (GRSF1))
- Autre désignation
- GRSF1 (GRSF1 Produits)
- Synonymes
- GRSF1 Protein, wu:fb62c04 Protein, zgc:153305 Protein, MGC186196 Protein, B130010H02 Protein, BB232551 Protein, C80280 Protein, D5Wsu31e Protein, G-rich RNA sequence binding factor 1 Protein, G-rich RNA sequence binding factor 1 S homeolog Protein, GRSF1 Protein, grsf1 Protein, grsf1.S Protein, Grsf1 Protein
- Sujet
- Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. {ECO:0000269|PubMed:23473033, ECO:0000269|PubMed:23473034}.
- Poids moléculaire
- 42.7 kDa Including tag.
- UniProt
- Q12849
-