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MAPRE1 Protein (AA 2-268) (His tag)

Crystallography grade MAPRE1 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3083387
  • Antigène Voir toutes MAPRE1 Protéines
    MAPRE1 (Microtubule-Associated Protein, RP/EB Family, Member 1 (MAPRE1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-268
    Origine
    • 5
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 3
    • 3
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette MAPRE1 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    AVNVYSTSVT SDNLSRHDML AWINESLQLN LTKIEQLCSG AAYCQFMDML FPGSIALKKV KFQAKLEHEY IQNFKILQAG FKRMGVDKII PVDKLVKGKF QDNFEFVQWF KKFFDANYDG KDYDPVAARQ GQETAVAPSL VAPALNKPKK PLTSSSAAPQ RPISTQRTAA APKAGPGVVR KNPGVGNGDD EAAELMQQVN VLKLTVEDLE KERDFYFGKL RNIELICQEN EGENDPVLQR IVDILYATDE GFVIPDEGGP QEEQEEY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human MAPRE1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product MAPRE1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    MAPRE1 (Microtubule-Associated Protein, RP/EB Family, Member 1 (MAPRE1))
    Autre désignation
    MAPRE1 (MAPRE1 Produits)
    Synonymes
    EB1 Protein, 5530600P05Rik Protein, AI462499 Protein, AI504412 Protein, AW260097 Protein, BIM1p Protein, D2Ertd459e Protein, Eb1 Protein, MAPRE1 Protein, mapre1l Protein, wu:fi33c06 Protein, zgc:55428 Protein, zgc:77807 Protein, mapre1 Protein, wu:fa01e12 Protein, wu:fc23e11 Protein, zgc:85755 Protein, MGC52508 Protein, eb1 Protein, xEB1B Protein, Mapre3 Protein, microtubule associated protein RP/EB family member 1 Protein, microtubule-associated protein, RP/EB family, member 1 Protein, microtubule-associated protein, RP/EB family, member 1a Protein, microtubule-associated protein, RP/EB family, member 1b Protein, microtubule associated protein RP/EB family member 1 L homeolog Protein, microtubule associated protein RP/EB family member 1 S homeolog Protein, MAPRE1 Protein, Mapre1 Protein, mapre1a Protein, mapre1b Protein, mapre1.L Protein, mapre1.S Protein
    Sujet
    Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. {ECO:0000269|PubMed:12388762, ECO:0000269|PubMed:16109370, ECO:0000269|PubMed:19632184, ECO:0000269|PubMed:21646404}.
    Poids moléculaire
    30.8 kDa Including tag.
    UniProt
    Q15691
    Pathways
    M Phase
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