MED30 Protein (AA 2-178) (His tag)

N° du produit ABIN3083616

Détail du produit

Antigène: MED30 (Mediator Complex Subunit 30 (MED30))

Type de proteíne: Recombinant

Attributs du protein: AA 2-178

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette MED30 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: STPPLAASGM APGPFAGPQA QQAAREVNTA SLCRIGQETV QDIVYRTMEI FQLLRNMQLP NGVTYHTGTY QDRLTKLQDN LRQLSVLFRK LRLVYDKCNE NCGGMDPIPV EQLIPYVEED GSKNDDRAGP PRFASEERRE IAEVNKKLKQ KNQQLKQIMD QLRNLIWDIN AMLAMRN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human MED30 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MED30 (Mediator Complex Subunit 30 (MED30))

Autre désignation: MED30 (MED30 Produits)

Synonymes: THRAP6 Protein, CG17183 Protein, Dmel\\CG17183 Protein, Med11/TRAP25 Protein, Med20 Protein, Med30 Protein, Trap25 Protein, dMED20 Protein, dTRAP25 Protein, med30 Protein, DDBDRAFT_0167644 Protein, DDBDRAFT_0266951 Protein, DDB_0167644 Protein, DDB_0266951 Protein, MED30S Protein, TRAP25 Protein, 1810038N03Rik Protein, 2510044J04Rik Protein, Thrap6 Protein, trap25 Protein, MGC154327 Protein, mediator complex subunit 30 Protein, Mediator complex subunit 30 Protein, mediator of RNA polymerase II transcription subunit 30 Protein, hypothetical protein Protein, mediator complex subunit 30 S homeolog Protein, MED30 Protein, med30 Protein, LOC412870 Protein, Med30 Protein, med30.S Protein

Sujet: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:11909976, ECO:0000269|PubMed:16595664}.

Poids moléculaire: 21.1 kDa Including tag.

UniProt: Q96HR3

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha