MEMO1 Protein (AA 1-297) (His tag)
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- Antigène Voir toutes MEMO1 Protéines
- MEMO1 (Mediator of Cell Motility 1 (MEMO1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-297
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Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette MEMO1 protéine est marqué à la His tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- Séquence
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MSNRVVCREA SHAGSWYTAS GPQLNAQLEG WLSQVQSTKR PARAIIAPHA GYTYCGSCAA HAYKQVDPSI TRRIFILGPS HHVPLSRCAL SSVDIYRTPL YDLRIDQKIY GELWKTGMFE RMSLQTDEDE HSIEMHLPYT AKAMESHKDE FTIIPVLVGA LSESKEQEFG KLFSKYLADP SNLFVVSSDF CHWGQRFRYS YYDESQGEIY RSIEHLDKMG MSIIEQLDPV SFSNYLKKYH NTICGRHPIG VLLNAITELQ KNGMNMSFSF LNYAQSSQCR NWQDSSVSYA AGALTVH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human MEMO1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product MEMO1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- MEMO1 (Mediator of Cell Motility 1 (MEMO1))
- Autre désignation
- MEMO1 (MEMO1 Produits)
- Synonymes
- MGC89105 Protein, C2orf4 Protein, MEMO Protein, NS5ATP7 Protein, 0610016J10Rik Protein, D930048L02Rik Protein, RGD1309929 Protein, fi04d12 Protein, wu:fi04d12 Protein, zgc:55290 Protein, mediator of cell motility 1 Protein, mediator of cell motility 1 L homeolog Protein, MEMO1 Protein, memo1 Protein, Memo1 Protein, memo1.L Protein
- Sujet
- May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration. {ECO:0000269|PubMed:15156151, ECO:0000269|PubMed:20937854}.
- Poids moléculaire
- 34.7 kDa Including tag.
- UniProt
- Q9Y316
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