Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

NAALAD2 Protein (AA 32-740) (His tag)

Crystallography grade NAALAD2 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3084180
  • Antigène Voir toutes NAALAD2 Protéines
    NAALAD2 (N-Acetylated alpha-Linked Acidic Dipeptidase 2 (NAALAD2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 32-740
    Origine
    • 3
    • 2
    Humain
    Source
    • 3
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette NAALAD2 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    KPLKETTTSV RYHQSIRWKL VSEMKAENIK SFLRSFTKLP HLAGTEQNFL LAKKIQTQWK KFGLDSAKLV HYDVLLSYPN ETNANYISIV DEHETEIFKT SYLEPPPDGY ENVTNIVPPY NAFSAQGMPE GDLVYVNYAR TEDFFKLERE MGINCTGKIV IARYGKIFRG NKVKNAMLAG AIGIILYSDP ADYFAPEVQP YPKGWNLPGT AAQRGNVLNL NGAGDPLTPG YPAKEYTFRL DVEEGVGIPR IPVHPIGYND AEILLRYLGG IAPPDKSWKG ALNVSYSIGP GFTGSDSFRK VRMHVYNINK ITRIYNVVGT IRGSVEPDRY VILGGHRDSW VFGAIDPTSG VAVLQEIARS FGKLMSKGWR PRRTIIFASW DAEEFGLLGS TEWAEENVKI LQERSIAYIN SDSSIEGNYT LRVDCTPLLY QLVYKLTKEI PSPDDGFESK SLYESWLEKD PSPENKNLPR INKLGSGSDF EAYFQRLGIA SGRARYTKNK KTDKYSSYPV YHTIYETFEL VEKFYDPTFK KQLSVAQLRG ALVYELVDSK IIPFNIQDYA EALKNYAASI YNLSKKHDQQ LTDHGVSFDS LFSAVKNFSE AASDFHKRLI QVDLNNPIAV RMMNDQLMLL ERAFIDPLGL PGKLFYRHII FAPSSHNKYA GESFPGIYDA IFDIENKANS RLAWKEVKKH ISIAAFTIQA AAGTLKEVL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NAALAD2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product NAALAD2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    NAALAD2 (N-Acetylated alpha-Linked Acidic Dipeptidase 2 (NAALAD2))
    Autre désignation
    NAALAD2 (NAALAD2 Produits)
    Synonymes
    2700022G20Rik Protein, D9Ertd285e Protein, Folh1b Protein, GCP3 Protein, GCPIII Protein, NAADALASE2 Protein, NAALADASE2 Protein, GPCIII Protein, NAALAD2 Protein, TRIM77 Protein, naalad2 Protein, N-acetylated alpha-linked acidic dipeptidase 2 Protein, N-acetylated-alpha-linked acidic dipeptidase 2 Protein, N-acetylated alpha-linked acidic dipeptidase 2 L homeolog Protein, Naalad2 Protein, NAALAD2 Protein, LOC485139 Protein, LOC100339446 Protein, naalad2.L Protein
    Sujet
    Has N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Also exhibits a dipeptidyl-peptidase IV type activity. Inactivate the peptide neurotransmitter N-acetylaspartylglutamate. {ECO:0000269|PubMed:10085079}.
    Poids moléculaire
    81.0 kDa Including tag.
    UniProt
    Q9Y3Q0
Vous êtes ici:
Support technique