Neurotensin Receptor 1 (High Affinity) (NTSR1) (AA 1-418) protein (rho-1D4 tag,His tag)

Détails pour le produit réf. ABIN3084600, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • NTSR1
  • Ntsr
  • NT-1R
  • NTR-1
  • NTR1
  • NTR
  • neurotensin receptor 1
  • neurotensin receptor 1 (high affinity)
  • NTSR1
  • Ntsr1
Attributs du protein
AA 1-418
1
Origine
Humain
3
2
Source
Cellules d'insectes
3
1
1
Type de proteíne
Recombinant
Purification/Conjugué
Cette NTSR1 protéine est marqué à la rho-1D4 tag,His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Fournisseur
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N° du produit (Fournisseur)
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Séquence HHHHHHRLNSSAPGT PGTPAADPFQ RAQAGLEEAL LAPGFGNASG NASERVLAAP SSELDVNTDI YSKVLVTAVY LALFVVGTVG NTVTAFTLAR KKSLQSLQST VHYHLGSLAL SDLLTLLLAM PVELYNFIWV HHPWAFGDAG CRGYYFLRDA CTYATALNVA SLSVERYLAI CHPFKAKTLM SRSRTKKFIS AIWLASALLA VPMLFTMGEQ NRSADGQHAG GLVCTPTIHT ATVKVVIQVN TFMSFIFPMV VISVLNTIIA NKLTVMVRQA AEQGQVCTVG GEHSTFSMAI EPGRVQALRH GVRVLRAVVI AFVVCWLPYH VRRLMFCYIS DEQWTPFLYD FYHYFYMVTN ALFYVSSTIN PILYNLVSAN FRHIFLATLA CLCPVWRRRR KRPAFSRKAD SVSSNHTLSS NATRETLYGSSGTETSQVAPA
Sequence including N-terminal His-tag and C-terminal Rho1D4 tag.
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human NTSR1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification The protein is purified from the cleared cell lysate using Rho1D4 capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Protein is endotoxin-free.
Classe de qualité Crystallography grade
Nom de la protéine
Sujet G-protein coupled receptor for the tridecapeptide neurotensin (NTS) (PubMed:8381365, PubMed:21725197, PubMed:23140271). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis (PubMed:21725197). {ECO:0000269|PubMed:21725197, ECO:0000269|PubMed:23140271, ECO:0000269|PubMed:8381365}.
Poids moléculaire 48.1 kDa Including tag.
UniProt P30989
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Commentaires

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer In solution (50 mM Hepes, pH8.0; 200 mM NaCl; 10% Glycerol; 0.1% n-Decyl-beta-maltoside; 0.01% cholesterolhemisuccinate; 0.01% CHAPSO)
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Images (Fournisseur)
 image for Neurotensin Receptor 1 (High Affinity) (NTSR1) (AA 1-418) protein (rho-1D4 tag,His tag) (ABIN3084600) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
 image for Neurotensin Receptor 1 (High Affinity) (NTSR1) (AA 1-418) protein (rho-1D4 tag,His tag) (ABIN3084600) Neurotensin Receptor 1 (High Affinity) (NTSR1) (AA 1-418) protein (rho-1D4 tag,His tag) (Image 2)
 image for Neurotensin Receptor 1 (High Affinity) (NTSR1) (AA 1-418) protein (rho-1D4 tag,His tag) (ABIN3084600) Neurotensin Receptor 1 (High Affinity) (NTSR1) (AA 1-418) protein (rho-1D4 tag,His tag) (Image 3)
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