Nucleobindin 1 Protein (NUCB1) (AA 27-461) (His tag)

N° du produit ABIN3084701

Détail du produit

Antigène: Nucleobindin 1 (NUCB1)

Type de proteíne: Recombinant

Attributs du protein: AA 27-461

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Nucleobindin 1 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: VPLERGAPNK EETPATESPD TGLYYHRYLQ EVIDVLETDG HFREKLQAAN AEDIKSGKLS RELDFVSHHV RTKLDELKRQ EVSRLRMLLK AKMDAEQDPN VQVDHLNLLK QFEHLDPQNQ HTFEARDLEL LIQTATRDLA QYDAAHHEEF KRYEMLKEHE RRRYLESLGE EQRKEAERKL EEQQRRHREH PKVNVPGSQA QLKEVWEELD GLDPNRFNPK TFFILHDINS DGVLDEQELE ALFTKELEKV YDPKNEEDDM REMEEERLRM REHVMKNVDT NQDRLVTLEE FLASTQRKEF GDTGEGWETV EMHPAYTEEE LRRFEEELAA REAELNAKAQ RLSQETEALG RSQGRLEAQK RELQQAVLHM EQRKQQQQQQ QGHKAPAAHP EGQLKFHPDT DDVPVPAPAG DQKEVDTSEK KLLERLPEVE VPQHL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NUCB1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Nucleobindin 1 (NUCB1)

Autre désignation: NUCB1 (NUCB1 Produits)

Synonymes: nuc Protein, MGC69294 Protein, MGC81496 Protein, NUCB1 Protein, zgc:153192 Protein, DKFZp459O1814 Protein, B230337F23Rik Protein, C77483 Protein, Calnuc Protein, MTEST82 Protein, Nucb Protein, CALNUC Protein, NUC Protein, nucleobindin 1 Protein, nucleobindin 1 S homeolog Protein, nucb1 Protein, nucb1.S Protein, NUCB1 Protein, Nucb1 Protein

Sujet: Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis (By similarity). {ECO:0000250}.

Poids moléculaire: 52.1 kDa Including tag.

UniProt: Q02818