CDC40 Protein (AA 1-579) (His tag)

N° du produit ABIN3086373

Détail du produit

Antigène: CDC40 (Cell Division Cycle 40 (CDC40))

Type de proteíne: Recombinant

Attributs du protein: AA 1-579

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette CDC40 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MSAAIAALAA SYGSGSGSES DSDSESSRCP LPAADSLMHL TKSPSSKPSL AVAVDSAPEV AVKEDLETGV HLDPAVKEVQ YNPTYETMFA PEFGPENPFR TQQMAAPRNM LSGYAEPAHI NDFMFEQQRR TFATYGYALD PSLDNHQVSA KYIGSVEEAE KNQGLTVFET GQKKTEKRKK FKENDASNID GFLGPWAKYV DEKDVAKPSE EEQKELDEIT AKRQKKGKQE EEKPGEEKTI LHVKEMYDYQ GRSYLHIPQD VGVNLRSTMP PEKCYLPKKQ IHVWSGHTKG VSAVRLFPLS GHLLLSCSMD CKIKLWEVYG ERRCLRTFIG HSKAVRDICF NTAGTQFLSA AYDRYLKLWD TETGQCISRF TNRKVPYCVK FNPDEDKQNL FVAGMSDKKI VQWDIRSGEI VQEYDRHLGA VNTIVFVDEN RRFVSTSDDK SLRVWEWDIP VDFKYIAEPS MHSMPAVTLS PNGKWLACQS MDNQILIFGA QNRFRLNKKK IFKGHMVAGY ACQVDFSPDM SYVISGDGNG KLNIWDWKTT KLYSRFKAHD KVCIGAVWHP HETSKVITCG WDGLIKLWD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CDC40 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CDC40 (Cell Division Cycle 40 (CDC40))

Autre désignation: CDC40 (CDC40 Produits)

Synonymes: DKFZp459B247 Protein, zgc:86860 Protein, T10O24.21 Protein, T10O24_21 Protein, An07g09150 Protein, AO090005001571 Protein, CDC40 Protein, Dmel\\CG6015 Protein, prp17 Protein, GB11047 Protein, PRP17 Protein, SLT15 Protein, SLU4 Protein, EHB3 Protein, PRPF17 Protein, 1200003H23Rik Protein, cell division cycle 40 Protein, hypothetical protein Protein, cell division cycle 40 homolog (S. cerevisiae) Protein, pre-mRNA-processing factor 17 Protein, Transducin/WD40 repeat-like superfamily protein Protein, splicing factor Prp17 Protein, Pre-mRNA-processing factor 17 Protein, CG6015 gene product from transcript CG6015-RA Protein, Cdc40p Protein, nuclear pre-mRNA splicing factor, component of the spliceosome Protein, mRNA splicing factor PRP17 Protein, CDC40 Protein, cdc40 Protein, CAALFM_C112750CA Protein, LOC100184816 Protein, LOC100636188 Protein, AT1G10580 Protein, ANI_1_1152064 Protein, AOR_1_2740174 Protein, CpipJ_CPIJ008035 Protein, CpipJ_CPIJ015768 Protein, SJAG_00639 Protein, PAAG_06400 Protein, MCYG_02443 Protein, VDBG_03904 Protein, MGYG_07449 Protein, Tsp_12048 Protein, CG6015 Protein, LOC413523 Protein, prp17 Protein, PRP17 Protein, Cdc40 Protein

Sujet: Associates with the spliceosome late in the splicing pathway and may function in the second step of pre-mRNA splicing. {ECO:0000269|PubMed:9830021}.

Poids moléculaire: 66.5 kDa Including tag.

UniProt: O60508

Pathways: Ribonucleoprotein Complex Subunit Organization